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Recombinant Human CD32b/FCGR2B Protein (HEK293 Cells, His Tag)

Uniprot : P31994

Cat.No.:PKSH031726
Expression host: HEK293 Cells

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100μg
1mg

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Description

Synonyms	Low Affinity Immunoglobulin Gamma Fc Region Receptor II-b;IgG Fc Receptor II-b;CDw32;Fc-Gamma RII-b;Fc-Gamma-RIIb;FcRII-b;CD32;FCGR2B;FCG2;IGFR2
Species	Human
Expression_host	HEK293 Cells
Sequence	Ala 46-Pro 217
Accession	NP_001002274.1
Application	Functional ELISA
Mol_Mass	20.8 kDa
AP_Mol_Mass	25-30 kDa
Tag	C-His
Bio_Activity	Immobilized recombinant human CD32b at 10 μg/ml (100 μl/well) can bind human IgG2 with a linear range of 0.16-6. 4 μg/ml.

Properties

Purity	> 95 % as determined by reducing SDS-PAGE.
Storage	Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80℃. Reconstituted protein solution can be stored at 4-8℃ for 2-7 days. Aliquots of reconstituted samples are stable at < -20℃ for 3 months.
Shipping	This product is provided as lyophilized powder which is shipped with ice packs.
Formulation	Lyophilized from sterile PBS, pH 7.4 Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization. Please refer to the specific buffer information in the printed manual.
Reconstitution	Please refer to the printed manual for detailed information.

Background

Processing of the N-terminal initiator methionine or formylated methionine is an essential cellular process conserved from prokaryotes to eukaryotes. The proteolytic removal of N-terminal methionine from nascent peptides is catalyzed by a family of enzymes known as methionine aminopeptidases (MetAPs) and is essential for cell growth. METAP1 and METAP2 have different substrate specificity due to the differences in both size and shape of the active sites. As a member of the M24 family of metalloproteases, METAP1 plays an important role in G(2)/M phase regulation of the cell cycle and may serve as a promising target for the discovery and development of new anticancer agents.

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