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Recombinant Human CD32b/FCGR2B Protein (His &AVI Tag), Biotinylated

Uniprot : P31994

Cat.No.:PKSH031724
Expression host: HEK293 Cells

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Description

Synonyms	Low Affinity Immunoglobulin Gamma Fc Region Receptor II-b;IgG Fc Receptor II-b;CDw32;Fc-Gamma RII-b;Fc-Gamma-RIIb;FcRII-b;CD32;FCGR2B;FCG2;IGFR2
Species	Human
Expression_host	HEK293 Cells
Sequence	Ala 46-Ile 224
Accession	NP_001002274.1
Application	Functional ELISA
Mol_Mass	24 kDa
Tag	C-His-Avi
Bio_Activity	Measured by its binding ability in a functional ELISA. Immobilized Human IgG1 at 10 μg/ml (100 μl/well) can bind Human CD32b. The EC50 of Human CD32b is 1. 6 - 3. 7 μg/ml. 2. Labeling ratio of biotin to protein: 0.5

Properties

Purity	> 95 % as determined by reducing SDS-PAGE.
Endotoxin level	< 1.0 EU per μg of the protein as determined by the LAL method.
Storage	Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80℃. Reconstituted protein solution can be stored at 4-8℃ for 2-7 days. Aliquots of reconstituted samples are stable at < -20℃ for 3 months.
Shipping	This product is provided as lyophilized powder which is shipped with ice packs.
Formulation	Lyophilized from sterile PBS, pH 7.4 Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization. Please refer to the specific buffer information in the printed manual.
Reconstitution	Please refer to the printed manual for detailed information.

Background

FcγRIIB is a low affinity receptor that recognizes the Fc portion of IgG. The human CD32 group consists of FcγRIIA, FcγRIIB, and FcγRIIC proteins that share 94-99% sequence identity in their extracellular domains but differ substantially in their transmembrane and cytoplasmic domains. FcγRII protein is expressed on cells of both myeloid and lymphoid lineages as well as on cells of non-hematopoietic origin. FcγRIIB has an intrinsic cytoplasmic immunoreceptor tyrosine-based inhibitory motif (ITIM) and delivers an inhibitory signal upon ligand binding. Ligation of FcγRIIB on B cells down-regulates antibody production and in some circumstances may promote apoptosis. Co-ligation of FcγRIIB on dendritic cells inhibits maturation and blocks cell activation. FcγRIIB may also be a target for monoclonal antibody therapy for malignancies. FcγRIIB plays an important negative-regulating role through modulating the signals from activation receptors.

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