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Recombinant Human CD4/LEU3 Protein (aa 1-208, His Tag)

Uniprot : P01730
  • Cat.No.:PKSH031631

  • Expression host: HEK293 Cells

To Purchase PKSH031631

Size:
  • 100μg
Price: $732
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Description

Synonyms T-cell surface glycoprotein CD4;T-cell surface antigen T4/Leu-3;CD4;Scd4;CD4mut
Species Human
Expression_host HEK293 Cells
Sequence Met 1-Ser 208
Accession NP_000607.1
Application Functional ELISA
Mol_Mass 21.7 kDa
AP_Mol_Mass 26 kDa
Tag C-His
Bio_Activity 1. Measured by its ability to bind biotinylated GP140-His in a functional ELISA.
2. Measured by the ability of the immobilized protein to support the adhesion of HeLa human cervical epithelial carcinoma cells. When 5 x 104 cells/well are added to CD4-coated plates (2. 5 μg/ml and 100 μl/well), approximately 40 %-60 % will adhere specifically after 60 minutes at 37℃.

Properties

Purity > 94 % as determined by reducing SDS-PAGE.
Endotoxin level < 1.0 EU per μg of the protein as determined by the LAL method.
Storage Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80℃. Reconstituted protein solution can be stored at 4-8℃ for 2-7 days. Aliquots of reconstituted samples are stable at < -20℃ for 3 months.
Shipping This product is provided as lyophilized powder which is shipped with ice packs.
Formulation Lyophilized from sterile PBS, pH 7.4
Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.
Please refer to the specific buffer information in the printed manual.
Reconstitution Please refer to the printed manual for detailed information.

Background

T-cell surface glycoprotein CD4, is a single-pass type I membrane protein. CD4 contains three Ig-like C2-type (immunoglobulin-like) domains and one Ig-like V-type (immunoglobulin-like) domain. CD4 is a glycoprotein expressed on the surface of T helper cells, regulatory T cells, monocytes, macrophages, and dendritic cells. The CD4 surface determinant, previously associated as a phenotypic marker for helper/inducer subsets of T lymphocytes, has now been critically identified as the binding/entry protein for human immunodeficiency viruses (HIV). The human CD4 molecule is readily detectable on monocytes, T lymphocytes, and brain tissues. CD4 is a co-receptor that assists the T cell receptor (TCR) to activate its T cell following an interaction with an antigen presenting cell. Using its portion that resides inside the T cell, CD4 amplifies the signal generated by the TCR. CD4 interacts directly with MHC class II molecules on the surface of the antigen presenting cell via its extracellular domain. CD4 is a primary receptor used by HIV-1 to gain entry into host T cells. HIV infection leads to a progressive reduction of the number of T cells possessing CD4 receptors. Viral protein U (VpU) of HIV-1 plays an important role in downregulation of the main HIV-1 receptor CD4 from the surface of infected cells. Physical binding of VpU to newly synthesized CD4 in the endoplasmic reticulum is an early step in a pathway leading to proteasomal degradation of CD4.

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