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Recombinant Human Cytochrome b5/CYB5A Protein (His Tag)

Uniprot : P00167

Cat.No.:PKSH032334
Expression host: E.coli

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Description

Synonyms	Cytochrome b5;Microsomal Cytochrome b5 Type A;MCB5;CYB5A;CYB5
Species	Human
Expression_host	E.coli
Sequence	Met 1-Asp134
Accession	P00167
Mol_Mass	17.5 kDa
AP_Mol_Mass	17 kDa
Tag	N-His

Properties

Purity	> 90 % as determined by reducing SDS-PAGE.
Endotoxin level	< 1.0 EU per μg of the protein as determined by the LAL method.
Storage	Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80℃. Reconstituted protein solution can be stored at 4-8℃ for 2-7 days. Aliquots of reconstituted samples are stable at < -20℃ for 3 months.
Shipping	This product is provided as lyophilized powder which is shipped with ice packs.
Formulation	Lyophilized from a 0.2 μm filtered solution of 20mM PB, 150mM NaCl, 0.1mM EDTA, pH 7.25. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization. Please refer to the specific buffer information in th
Reconstitution	Please refer to the printed manual for detailed information.

Background

Cytochrome b5 (CYB5A) is a membrane bound hemoprotein which function as an electron carrier for several membrane bound oxygenases. CYB5A contains one cytochrome b5 heme-binding domain and has two isoforms produced by alternative splicing. Isoform 1 is a sngle-pass membrane protein. Isoform 2 is located in cytoplasm. The defects in CYB5A can result in type IV hereditary methemoglobinemia.

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