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Cat.No.:PKSH031764
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Synonyms | Cadherin-1;CDH1;CAM 120/80;E-cadherin;CD324;CDHE;E-cad;E-Cadherin;ECAD;LCAM;UVO;Arc-1 |
Species | Human |
Expression_host | HEK293 Cells |
Sequence | Met 1-Ile707 |
Accession | P12830 |
Mol_Mass | 87.1 kDa |
AP_Mol_Mass | 116-126 kDa |
Tag | C-hFc |
Bio_Activity | Measured by the ability of the immobilized protein to support the adhesion of MCF-7 human breast adenocarcinoma cells. When cells are added to E-Cad coated plates (5 μg/mL, 100 μL/well), approximately 33% will adhere specifically after 90 minutes at 37 ℃. |
Purity | > 90 % as determined by reducing SDS-PAGE. |
Endotoxin level | < 1.0 EU per μg of the protein as determined by the LAL method. |
Storage | Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80℃. Reconstituted protein solution can be stored at 4-8℃ for 2-7 days. Aliquots of reconstituted samples are stable at < -20℃ for 3 months. |
Shipping | This product is provided as lyophilized powder which is shipped with ice packs. |
Formulation | Lyophilized from sterile PBS, pH 7.4 Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization. Please refer to the specific buffer information in the printed manual. |
Reconstitution | Please refer to the printed manual for detailed information. |