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Cat.No.:PKSH031504
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Synonyms | Serine protease HTRA2;mitochondrial;High temperature requirement protein A2;HtrA2;Omi stress-regulated endoprotease;Serine protease 25;Serine proteinase OMI;HTRA2;OMI;PRSS25 |
Species | Human |
Expression_host | E.coli |
Sequence | Ala 134-Glu 458 |
Accession | O43464-1 |
Mol_Mass | 36.5 kDa |
AP_Mol_Mass | 36.5 kDa |
Tag | C-His |
Bio_Activity | Protease activity demonstrated by HtrA2 cleavage of bovine β-casein (Sigma, Catalog # C-6905). Incubation of β-casein at 0.2 mg/mL with Recombinant Human HTRA-2 at 0.02 mg/mL (ratio of 10:1) for 60 minutes at 45℃ in 50 mM Tris, pH 8.0, which results in > 95% cleavage of β-casein, as revealed by SDS-PAGE. |
Purity | > 87 % as determined by reducing SDS-PAGE. |
Endotoxin level | Please contact us for more information. |
Storage | Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80℃. Reconstituted protein solution can be stored at 4-8℃ for 2-7 days. Aliquots of reconstituted samples are stable at < -20℃ for 3 months. |
Shipping | This product is provided as lyophilized powder which is shipped with ice packs. |
Formulation | Lyophilized from sterile 50mM Tris, 0.3M NaCl, 1mM DTT, 20% Glycerol, pH 7.8 Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization. Please refer to the specific buffer information in the printed ma |
Reconstitution | Please refer to the printed manual for detailed information. |