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Cat.No.:PKSH031437
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Synonyms | Kallikrein-11;hK11;Hippostasin;Serine Protease 20;Trypsin-Like Protease;KLK11;PRSS20;TLSP |
Species | Human |
Expression_host | HEK293 Cells |
Sequence | Met 1-Asn 250 |
Accession | NP_006844.1 |
Mol_Mass | 27.0 kDa |
AP_Mol_Mass | 40 kDa |
Tag | C-His |
Bio_Activity | Measured by its ability to cleave a colorimetric peptide substrate D-Val-Leu-Lys-ThioBenzyl ester (VLK-SBzl), in the presence of 5, 5'Dithio-bis (2-nitrobenzoic acid) (DTNB) (Edwards, K. M. et al. , 1999, J. Biol. Chem. 274: 30468). The specific activity is > 200 pmoles/min/μg. (Activation description: The proenzyme needs to be activated by Thermolysin for an activated form) |
Purity | > 90 % as determined by reducing SDS-PAGE. |
Endotoxin level | < 1.0 EU per μg of the protein as determined by the LAL method. |
Storage | Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80℃. Reconstituted protein solution can be stored at 4-8℃ for 2-7 days. Aliquots of reconstituted samples are stable at < -20℃ for 3 months. |
Shipping | This product is provided as lyophilized powder which is shipped with ice packs. |
Formulation | Lyophilized from sterile PBS, pH 7.4 Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization. Please refer to the specific buffer information in the printed manual. |
Reconstitution | Please refer to the printed manual for detailed information. |