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Recombinant Human Thymidylate Kinase/DTYMK Protein (His Tag)

Uniprot : P23919

Cat.No.:PKSH033115
Expression host: HEK293 Cells

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Description

Synonyms	Thymidylate kinase;dTMP kinase;DTYMK;CDC8;TMPK;TYMK
Species	Human
Expression_host	HEK293 Cells
Sequence	Met 1-Lys212
Accession	P23919
Mol_Mass	24.9 kDa
AP_Mol_Mass	25-31 kDa
Tag	C-His

Properties

Purity	> 95 % as determined by reducing SDS-PAGE.
Endotoxin level	< 1.0 EU per μg of the protein as determined by the LAL method.
Storage	Store at < -20°C, stable for 6 months. Please minimize freeze-thaw cycles.
Shipping	This product is provided as liquid. It is shipped at frozen temperature with blue ice/gel packs. Upon receipt, store it immediately at < - 20°C.
Formulation	Supplied as a 0.2 μm filtered solution of 20mM PB, 150mM NaCl, 10% Sucrose, 0.05% Tween 80, 20% Glycerol, 1mM EDTA, pH8.0.
Reconstitution	Not Applicable

Background

Thymidylate kinase (DTYMK) is a ubiquitous enzyme of about 25 kD which belongs to thymidylate kinase family. DTYMK is important in the dTTP synthesis pathway for DNA synthesis. It participated in the pyrimidine metabolism pathway and dTTP biosynthesis pathway. DTYMK catalyzes the conversion of dTMP to dTDP and catalyzes the phosphorylation of thymidine 5'-monophosphate (dTMP) to form thymidine 5'-diphosphate (dTDP) in the presence of ATP and magnesium. Structural and functional analyses suggest that the cDNA codes for authentic human dTMP kinase. The mRNA levels and enzyme activities corresponded to cell cycle progression and cell growth stages.

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