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Recombinant Mouse Carbonic Anhydrase 4/CA4 Protein (His Tag)

Uniprot : Q64444
  • Cat.No.:PKSM040721

  • Expression host: HEK293 Cells

To Purchase PKSM040721

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  • 50μg
Price: $721
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Description

Synonyms CA4;CAIV;CA-IV;Car4;Carbonate dehydratase IV;carbonic anhydrase 4;carbonic anhydrase IVRP17;carbonic dehydratase IV;EC4.2.1.1;retinitis pigmentosa 17;RP17
Species Mouse
Expression_host HEK293 Cells
Sequence Met 1-Ser 277
Accession NP_031633.1
Mol_Mass 31.0 kDa
AP_Mol_Mass 31 kDa
Tag C-His
Bio_Activity Measured by its esterase activity. The specific activity is > 10 pmoles/min/μg.

Properties

Purity > 98 % as determined by reducing SDS-PAGE.
Endotoxin level < 1.0 EU per μg of the protein as determined by the LAL method.
Storage Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80℃. Reconstituted protein solution can be stored at 4-8℃ for 2-7 days. Aliquots of reconstituted samples are stable at < -20℃ for 3 months.
Shipping This product is provided as lyophilized powder which is shipped with ice packs.
Formulation Lyophilized from sterile PBS, pH 7.4
Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.
Please refer to the specific buffer information in the printed manual.
Reconstitution Please refer to the printed manual for detailed information.

Background

The carbonic anhydrases (or carbonate dehydratases) are classified as metalloenzyme for its zinc ion prosthetic group and form a family of enzymes that catalyze the rapid interconversion of carbon dioxide and water to bicarbonate and protons, a reversible reaction that takes part in maintaining acid-base balance in blood and other tissues. The carbonic anhydrasekl (CA) family consists of at least 11 enzymatically active members and a few inactive homologous proteins. Carbonic anhydrase IV (CAIV) is a membrane-associated enzyme anchored to plasma membrane surfaces by a phosphatidylinositol glycan linkage. CAIV is a high-activity isozyme in CO2 hydration comparable to that of CAII. Furthermore, CAIV is more active in HCO3- dehydration than is CAII. However, the esterase activity of CAIV is decreased 150-fold compared to CAII.

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