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Recombinant Mouse Carbonic Anhydrase II/CA2 Protein (His Tag)

Uniprot : P00920
  • Cat.No.:PKSM040550

  • Expression host: E.coli

To Purchase PKSM040550

Size:
  • 100μg
  • 1mg
Price: $670
Qty:

Description

Synonyms AI131712;Ca2;CAII;Car-2;Ltw-5;Lvtw-5
Species Mouse
Expression_host E.coli
Sequence Met 1-Lys 260
Accession AAH55291.1
Mol_Mass 30.4 kDa
AP_Mol_Mass 30-33 kDa
Tag C-His
Bio_Activity Measured by its esterase activity. The specific activity is > 100 pmoles/min/μg.

Properties

Purity > 96 % as determined by reducing SDS-PAGE.
Endotoxin level Please contact us for more information.
Storage Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80℃. Reconstituted protein solution can be stored at 4-8℃ for 2-7 days. Aliquots of reconstituted samples are stable at < -20℃ for 3 months.
Shipping This product is provided as lyophilized powder which is shipped with ice packs.
Formulation Lyophilized from sterile 50mM Tris, 150mM NaCl, pH 7.5
Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.
Please refer to the specific buffer information in the printed manual.
Reconstitution Please refer to the printed manual for detailed information.

Background

The carbonic anhydrases (or carbonate dehydratases) are classified as metalloenzyme for its zinc ion prosthetic group and form a family of enzymes that catalyze the rapid interconversion of carbon dioxide and water to bicarbonate and protons, a reversible reaction that takes part in maintaining acid-base balance in blood and other tissues. The carbonic anhydrasekl (CA) family consists of at least 11 enzymatically active members and a few inactive homologous proteins. Carbonic anhydrase II is one of fourteen forms of human α carbonic anhydrases. Defects in this enzyme are associated with osteopetrosis and renal tubular acidosis. Renal carbonic anhydrase allows the reabsorption of sodium ions in the proximal tubule. Carbonic anhydrase II has been shown to interact with Band 3 and Sodium-hydrogen antiporter 1.

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