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Recombinant Mouse Carbonic Anhydrase VIII/CA8 Protein (His Tag)

Uniprot : P28651
  • Cat.No.:PKSM040495

  • Expression host: E.coli

To Purchase PKSM040495

Size:
  • 100μg
Price: $855
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Description

Synonyms AW546993;Ca8;Cals;Cals1;Car8;Carp;RP23-180H12.1;wdl
Species Mouse
Expression_host E.coli
Sequence Met 1-Gln 291
Accession P28651
Mol_Mass 34.5 kDa
AP_Mol_Mass 37 kDa
Tag C-His
Bio_Activity Measured by its esterase activity. The specific activity is > 5 pmoles/min/μg.

Properties

Purity > 88 % as determined by reducing SDS-PAGE.
Endotoxin level Please contact us for more information.
Storage Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80℃. Reconstituted protein solution can be stored at 4-8℃ for 2-7 days. Aliquots of reconstituted samples are stable at < -20℃ for 3 months.
Shipping This product is provided as lyophilized powder which is shipped with ice packs.
Formulation Lyophilized from sterile 50mM Tris, pH 8.0
Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.
Please refer to the specific buffer information in the printed manual.
Reconstitution Please refer to the printed manual for detailed information.

Background

The carbonic anhydrases (or carbonate dehydratases) are classified as metalloenzyme for its zinc ion prosthetic group and form a family of enzymes that catalyze the rapid interconversion of carbon dioxide and water to bicarbonate and protons, a reversible reaction that takes part in maintaining acid-base balance in blood and other tissues. The carbonic anhydrasekl (CA) family consists of at least 11 enzymatically active members and a few inactive homologous proteins. Carbonic anhydrase protein (CA) VIII, which is a member of the CA gene family, has been shown to have no catalytic CA activity and its biological function is still unknown. Increased expression of CA-RP VIII was observed in 78% of colorectal carcinomas. It suggested that CA-RP VIII plays a role in the process of invasion in colorectal cancer.

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