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Recombinant Mouse Coagulation Factor X/F10 Protein (His Tag)

Uniprot : O88947
  • Cat.No.:PKSM040986

  • Expression host: HEK293 Cells

To Purchase PKSM040986

Size:
  • 10μg
  • 50μg
Price: $178
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Description

Synonyms F10;Coagulation factor X;Stuart factor
Species Mouse
Expression_host HEK293 Cells
Sequence Gly21-Asn481
Accession O88947
Mol_Mass 34.6&18.4 kDa
AP_Mol_Mass 50-60&20-28 kDa
Tag C-His

Properties

Purity > 95 % as determined by reducing SDS-PAGE.
Endotoxin level < 1.0 EU per μg of the protein as determined by the LAL method.
Storage Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80℃. Reconstituted protein solution can be stored at 4-8℃ for 2-7 days. Aliquots of reconstituted samples are stable at < -20℃ for 3 months.
Shipping This product is provided as lyophilized powder which is shipped with ice packs.
Formulation Lyophilized from a 0.2 μm filtered solution of 20mM MES, 150mM NaCl, 1mM CaCl2, pH 7.5.
Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.
Please refer to the specific buffer informa
Reconstitution Please refer to the printed manual for detailed information.

Background

Mouse coagulation factor X / F10 a member of the peptidase S1 family. The mature F10 is composed mostly of two EGF-like domains, one Gla gamma-carboxy-glutamate domain and one peptidase S1 domain. Factor Xa is a vitamin K-dependent plasma protease that converts prothrombin to thrombin in the presence of factor Va, calcium and phospholipid during blood clotting. The two chains of F10 are formed from a single-chain precursor by the excision of two Arg residues. A single-chain precursor is initially synthesized in the liver. The light and heavy chains are linked together by disulfide bonds. The light chain contains a Gla and two EGF-like domains. The heavy chain corresponds to the serine protease domain. It can form a heterodimer with SERPINA5.

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