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Recombinant Mouse Kallikrein 1/KLK1 Protein (His Tag)

Uniprot : P15947

Cat.No.:PKSM040442
Expression host: HEK293 Cells

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50μg

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Description

Synonyms	Glandular kallikrein K1;KAL-B;Renal kallikrein;Tissue kallikrein-6;mGK-6
Species	Mouse
Expression_host	HEK293 Cells
Sequence	Met1-Asp261
Accession	P15947
Mol_Mass	28.3 kDa
AP_Mol_Mass	36 kDa
Tag	C-His
Bio_Activity	Measured by its ability to cleave a flourogenic peptide substrate Pro-Phe-Arg-7-amido-4-methylcoumarin(PFR-AMC). The specific activity is > 6, 000 pmol/min/μg. (Activation description: The proenzyme needs to be activated by Thermolysin for an activated form)

Properties

Purity	> 90 % as determined by reducing SDS-PAGE.
Endotoxin level	< 1.0 EU per μg of the protein as determined by the LAL method.
Storage	Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80℃. Reconstituted protein solution can be stored at 4-8℃ for 2-7 days. Aliquots of reconstituted samples are stable at < -20℃ for 3 months.
Shipping	This product is provided as lyophilized powder which is shipped with ice packs.
Formulation	Lyophilized from sterile 25 mM Tris, 5 mM CaCl2, 0.15 M NaCl, pH 7.4 Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization. Please refer to the specific buffer information in the printed manual.
Reconstitution	Please refer to the printed manual for detailed information.

Background

Human tissue kallikrein (KLK1) is a serine protease, component of the KKS that has been demonstrated to exert pleiotropic beneficial effects in protection from tissue injury through its antiinflammatory, antiapoptotic, antifibrotic and antioxidative actions.Polymorphism of the human tissue kallikrein 1 (KLK1) A1789G gene is associated with susceptibility to hypertension.

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