Synonyms	Renin-1;Angiotensinogenase;Kidney renin;Ren1;Ren;Ren-1;Angiotensin-forming enzyme;Ren-A;Ren1c;Ren1d;Rn-1;Rnr
Species	Mouse
Expression_host	HEK293 Cells
Sequence	Met 1-Arg 402
Accession	NP_112469.1
Application	Functional ELISA
Mol_Mass	43.2 kDa
AP_Mol_Mass	48-55 kDa
Tag	C-His
Bio_Activity	Measured by its ability to cleave the fluorogenic peptide substrate 5FAM/QXL™ 520 (PetiPeterdi, J. et al., 2009, Physiology 24:88.). The specific activity is > 20 pmoles/min/μg. 2. Immobilized mouse REN1-His at 10ug/ml (100 μl/well) can bind biotinylated human AGT-His with a linear range of 31. 25-250 ng/ml.

Properties

Purity	> 97 % as determined by reducing SDS-PAGE.
Endotoxin level	< 1.0 EU per μg of the protein as determined by the LAL method.
Storage	Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80℃. Reconstituted protein solution can be stored at 4-8℃ for 2-7 days. Aliquots of reconstituted samples are stable at < -20℃ for 3 months.
Shipping	This product is provided as lyophilized powder which is shipped with ice packs.
Formulation	Lyophilized from sterile PBS, pH 7.4 Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization. Please refer to the specific buffer information in the printed manual.
Reconstitution	Please refer to the printed manual for detailed information.

Background

Renin-1, also known as Ren-1, Angiotensinogenase and Kidney renin, is a member of thepeptidase A1 family. Renin-1 is synthesized by the juxtaglomerular cells of the kidney in response to decreased blood pressure and sodium concentration. androgen and thyroid hormones influence levels of Renin-1 in mouse submandibular gland (SMG) primarily by regulating the amount of Renin-1 mRNA available for translation. Renin-1 is a highly specific endopeptidase, whose only known function is to generate angiotensin I from angiotensinogen in the plasma, initiating a cascade of reactions that produce an elevation of blood pressure and increased sodium retention by the kidney. It is expressed at relatively low levels in mouse SMG and kidney. Ren-2 is expressed at high levels in the mouse SMG and at very low levels, if at all, in the kidney. Ren-1 and Ren-2 are closely linked on mouse chromosome 1, show extensive homology in coding and noncoding regions and provide a model for studying the regulation of gene expression.

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