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Cat.No.:PKSM040504
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Synonyms | A-SMase;ASM;aSMase;Zn-SMase |
Species | Mouse |
Expression_host | Baculovirus-Insect Cells |
Sequence | Met 1-Leu 626 |
Accession | Q04519 |
Mol_Mass | 66.3 kDa |
AP_Mol_Mass | 63 kDa |
Tag | C-His |
Bio_Activity | Measured by its ability to cleave 2-N-Hexadecanoylamino-4-nitrophenylphosphorylcholine(HNPPC). The specific activity is > 1, 500 pmoles/min/μg. |
Purity | > 85 % as determined by reducing SDS-PAGE. |
Endotoxin level | < 1.0 EU per μg of the protein as determined by the LAL method. |
Storage | Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80℃. Reconstituted protein solution can be stored at 4-8℃ for 2-7 days. Aliquots of reconstituted samples are stable at < -20℃ for 3 months. |
Shipping | This product is provided as lyophilized powder which is shipped with ice packs. |
Formulation | Lyophilized from sterile 20mM Tris, 500mM NaCl, 10% glycerol, pH 8.0, 0.1% Tween20 Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization. Please refer to the specific buffer information in the pri |
Reconstitution | Please refer to the printed manual for detailed information. |