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Cat.No.:PKSM040357
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Synonyms | Sonic Hedgehog Protein;SHH;HHG-1;SHH;9530036O11Rik;Dsh;Hhg1;Hx;Hxl3;M10008 |
Species | Mouse |
Expression_host | HEK293 Cells |
Sequence | Met1-Gly198 |
Accession | Q62226 |
Mol_Mass | 21 kDa |
Tag | C-His |
Bio_Activity | Measured by its ability to induce alkaline phosphatase production by C3H10T1/2 mouse embryonic fibroblast cells. The ED50 for this effect is typically 5-40 μg/mL. |
Purity | > 90 % as determined by reducing SDS-PAGE. |
Endotoxin level | < 1.0 EU per μg of the protein as determined by the LAL method. |
Storage | Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80℃. Reconstituted protein solution can be stored at 4-8℃ for 2-7 days. Aliquots of reconstituted samples are stable at < -20℃ for 3 months. |
Shipping | This product is provided as lyophilized powder which is shipped with ice packs. |
Formulation | Lyophilized from sterile PBS, pH 7.4 Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization. Please refer to the specific buffer information in the printed manual. |
Reconstitution | Please refer to the printed manual for detailed information. |