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Cat.No.:PKSR030241
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Synonyms | CD6 |
Species | Rat |
Expression_host | HEK293 Cells |
Sequence | Met1-Asn396 |
Accession | Q812A4 |
Application | Functional ELISA |
Mol_Mass | 42.5 kDa |
AP_Mol_Mass | 64-70 kDa |
Tag | C-His |
Bio_Activity | 1. Immobilized rat CD6-His at 10 μg/mL (100 μL/well) can bind rat ALCAM-Fc3, The EC50 of rat ALCAM-Fc3 is 19.5-45. 5 ng/ml. 2. Measured by the ability of the immobilized protein to support the adhesion of Jurkat human acute T cell leukemia cells. When 8 x 104cells/well are added to rat CD6-His coated plates (10μg/mL, 100 μL/well), approximately 39.2% will adhere after 60 minutes at 37℃. |
Purity | > 95 % as determined by reducing SDS-PAGE. |
Endotoxin level | < 1.0 EU per μg of the protein as determined by the LAL method. |
Storage | Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80℃. Reconstituted protein solution can be stored at 4-8℃ for 2-7 days. Aliquots of reconstituted samples are stable at < -20℃ for 3 months. |
Shipping | This product is provided as lyophilized powder which is shipped with ice packs. |
Formulation | Lyophilized from sterile PBS, pH 7.4 Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization. Please refer to the specific buffer information in the printed manual. |
Reconstitution | Please refer to the printed manual for detailed information. |