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Cat.No.:PKSR030387
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Synonyms | SELE;Elam-1;CD62e |
Species | Rat |
Expression_host | HEK293 Cells |
Sequence | Met 1-Pro 494 |
Accession | P98105-1 |
Mol_Mass | 53.0 kDa |
AP_Mol_Mass | 70-80 kDa |
Tag | C-His |
Bio_Activity | Measured by the ability of the immobilized protein to support the adhesion of U937 human histiocytic lymphoma cells. When 5 x 104 cells/well are added to rat E Selectin/Fc Chimera coated plates (2 μg/mL, 100 μL/well), approximately 30%-60% will adhere after 1 hour at 37℃. Optimal dilutions should be determined by each laboratory for each application. |
Purity | > 97 % as determined by reducing SDS-PAGE. |
Endotoxin level | < 1.0 EU per μg of the protein as determined by the LAL method. |
Storage | Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80℃. Reconstituted protein solution can be stored at 4-8℃ for 2-7 days. Aliquots of reconstituted samples are stable at < -20℃ for 3 months. |
Shipping | This product is provided as lyophilized powder which is shipped with ice packs. |
Formulation | Lyophilized from sterile PBS, pH 7.4 Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization. Please refer to the specific buffer information in the printed manual. |
Reconstitution | Please refer to the printed manual for detailed information. |