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Cat.No.:PKSV030392
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Synonyms | coronavirus s1 Protein;SARS-CoV-;coronavirus s2 Protein |
Species | SARS-CoV-2 |
Expression_host | Baculovirus-Insect Cells |
Sequence | Met1-Pro1213 |
Accession | YP_009724390.1 |
Mol_Mass | 134.3 kDa |
Tag | C-His |
Bio_Activity | Measured by its binding ability in a functional ELISA. Immobilized human ACE2 protein (Fc tag)(PKSH031870) at 2 μg/mL (100 μL/well) can bind SARS-CoV-2 (2019-nCoV) Spike S1+S2 ECD (D614G)-His(PKSV030392), the EC50 of PKSV030392 is 0.5-1. 2 μg/mL. |
Purity | > 90 % as determined by reducing SDS-PAGE. |
Endotoxin level | < 1.0 EU per μg of the protein as determined by the LAL method. |
Storage | Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80℃. Reconstituted protein solution can be stored at 4-8℃ for 2-7 days. Aliquots of reconstituted samples are stable at < -20℃ for 3 months. |
Shipping | This product is provided as lyophilized powder which is shipped with ice packs. |
Formulation | Lyophilized from sterile 20 mM Tris, 300 mM NaCl, 10 % glycerol, pH 8.0. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization. Please refer to the specific buffer information in the printed manual |
Reconstitution | Please refer to the printed manual for detailed information. |