Current Position:Home>>Kits>>Metabolism Assays>> Sialic Acid (SA) Colorimetric Assay Kit

Sialic Acid (SA) Colorimetric Assay Kit

Cat.No.:E-BC-K068-M
Detection instrument: Microplate reader(510-570 nm,optimum wavelength: 560 nm)

Detection method: Colorimetric method

To Purchase E-BC-K068-M

Size:

96T
48T

Price: $200

Qty:

Welcome to order from local distributors.

Protocols for metabolism assays

FAQ for metabolism assays

Have Questions?

Add to cart Bulk request

For research use only. Order now, ship in 3 days

Detection principle

Sialic acid forms a purplish red complex with methyl resorcinol in the presence of oxidant. And the absorbance conforms to Lambert-Beer's law. The content of sialic acid can be calculated by measuring the OD value at 560 nm.

Performance characteristics

Synonyms	SA
Sample type	Serum,plasma,saliva,urine,hydrothorax,tissue
Sensitivity	0.03 mmol/L
Detection range	0.03-7 mmol/L
Detection method	Colorimetric method
Assay type	Quantitative
Assay time	40 min
Precision	Average inter-assay CV: 6.100%Average intra-assay CV: 4%
Other instruments required	Micropipettor, Centrifuge, Incubator, Water bath, Vortex mixer
Other reagents required	Normal saline (0.9% NaCl), PBS (0.01 M, pH 7.4)
Storage	Reagent 1: -20℃, Reagent 2: 2-8℃
Valid period	12 months

Dilution of sample

It is recommended to take 2~3 samples with expected large difference to do pre-experiment before formal experiment and dilute the sample according to the result of the pre-experiment and the detection range (0.03-7 mmol/L).

The recommended dilution factor for different samples is as follows (for reference only):

Sample type	Dilution factor
Human serum	1
Rat serum	1
Mouse serum	1
Porcine serum	1
Human plasma	1
Rat plasma	1
Mouse plasma	1
Human saliva	1
Human urine	1
Human hydrothorax	1
10% Plant tissue homogenate	1
10% Mouse heart tissue homogenate	1
10% Mouse liver tissue homogenate	1
10% Mouse kidney tissue homogenate	1
10% Mouse lung tissue homogenate	1
10% Mouse brain tissue homogenate	1

Note: The diluent is normal saline (0.9% NaCl) or PBS (0.01 M, pH 7.4).

Troubleshooting

Collection and treatment of common samples for metabolism assay detection

Questions and Answers of Metabolism Assay Kits

Citations

Reviews/Q&A

Show all
Reviews
Q&A

... Show All Show Less

Cat. No	E-BC-K068-M
Application
Sample Type

*Product Name:
*Catalog Number:
*Name:
*Email:
*Message:
*Country:
*When will you use it?	Within 1 month In 2 to 3 month In 4 to 6 month 6 months later
*Captcha:	Click to load Captcha