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Sialic Acid (SA) Colorimetric Assay Kit

  • Cat.No.:E-BC-K068-M

  • Detection instrument: Microplate reader(510-570 nm,optimum wavelength: 560 nm)

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  • 48T
Price: $200
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Detection principle

Sialic acid forms a purplish red complex with methyl resorcinol in the presence of oxidant. And the absorbance conforms to Lambert-Beer's law. The content of sialic acid can be calculated by measuring the OD value at 560 nm.

Performance characteristics

Synonyms SA
Sample type Serum,plasma,saliva,urine,hydrothorax,tissue
Sensitivity 0.03 mmol/L
Detection range 0.03-7 mmol/L
Detection method Colorimetric method
Assay type Quantitative
Assay time 40 min
Precision Average inter-assay CV: 6.1%Average intra-assay CV: 4%
Other instruments required Micropipettor, Centrifuge, Incubator, Water bath, Vortex mixer
Other reagents required Normal saline (0.9% NaCl), PBS (0.01 M, pH 7.4)
Storage Reagent 1: -20℃, Reagent 2: 2-8℃
Valid period 6 months

Dilution of sample

It is recommended to take 2~3 samples with expected large difference to do pre-experiment before formal experiment and dilute the sample according to the result of the pre-experiment and the detection range (0.03-7 mmol/L).

The recommended dilution factor for different samples is as follows (for reference only):

Sample type

Dilution factor

Human serum

1

Rat serum

1

Mouse serum

1

Porcine serum

1

Human plasma

1

Rat plasma

1

Mouse plasma

1

Human saliva

1

Human urine

1

Human hydrothorax

1

10% Plant tissue homogenate

1

10% Mouse heart tissue homogenate

1

10% Mouse liver tissue homogenate

1

10% Mouse kidney tissue homogenate

1

10% Mouse lung tissue homogenate

1

10% Mouse brain tissue homogenate

1

Note: The diluent is normal saline (0.9% NaCl) or PBS (0.01 M, pH 7.4).

Citations

From now on, if you have published a paper by using any of our products since 1/1/2019, fill out the “Elabscience Publication Reward Application Form”carefully and send it to orders@elabscience.com, we will get back to you with the reward after we confirm it ASAP!

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