SDH catalyzes the dehydrogenation of succinate to fumarate, with electron transport materials transferring electrons to 2, 6-dichlorophenol indiophenol (DCPIP). Then, the reduced DCPIP is reduced to the oxidized DCPIP, which has a characteristic absorption peak at 600 nm. Therefore, the activity of SDH can be quantified by measure the change OD value at 600 nm.

Performance characteristics

Synonyms	SDH
Sample type	animal tissue, cell
Sensitivity	0.83 U/L
Detection range	0.83-65.42 U/L
Detection method	Colorimetric method
Assay type	Enzyme Activity
Assay time	10 min
Precision	Average inter-assay CV: 4%Average intra-assay CV: 2%
Other instruments required	Centrifuge
Other reagents required	Normal saline (0.9% NaCl), PBS(0.01 M，pH 7.4).
Storage	-20℃
Valid period	12 months

Troubleshooting

Collection and treatment of common samples for metabolism assay detection

Questions and Answers of Metabolism Assay Kits

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Cat. No	E-BC-K649-M
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