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Current Position:Home>>Kits>>Metabolism Assays>> Total Antioxidant Capacity (T-AOC) Colorimetric Assay Kit (ABTS, Enzyme Method)

Total Antioxidant Capacity (T-AOC) Colorimetric Assay Kit (ABTS, Enzyme Method)

Citations(29)

  • Cat.No.:E-BC-K219-M

  • Detection instrument: Microplate reader(405-425 nm)

Manual MSDS

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Detection principle

The principle of the ABTS method for determining the T-AOC is as follows. ABTS is oxidized to green ABTS+ by appropriate oxidant, which can be inhibited if there exist antioxidants. The T-AOC of the sample can be determined and calculated by measuring the absorbance of ABTS+ at 414 nm or 734 nm. Trolox is an analog of VE and has a similar antioxidant capacity to that of VE. Trolox is used as a reference for other antioxidant antioxidants. For example, the T-AOC of Trolox is 1, then the antioxidant capacity of the other substance with the same concentration is showed by the ratio of its antioxidant capacity to Trolox antioxidant capacity.

Performance characteristics

Synonyms T-AOC
Sample type Serum,plasma,urine,saliva,tissue,cells
Sensitivity 0.047 mmol/L
Detection range 0.047-1.50 mmol/L
Detection method Colorimetric method
Assay type Quantitative
Assay time 20 min
Precision Average inter-assay CV: 4.100%Average intra-assay CV: 2.200%
Other instruments required Micropipettor
Other reagents required Normal saline (0.9% NaCl), PBS (0.01 M, pH 7.4), 60% Ethanol
Storage Reagent5:-20℃,Others:2-8℃
Valid period 12 months

Images

T-AOC was significantly increased in C-RIS Group (light green bar). (P<0.05)

J A Garcia-Partida et al investigate the effect of mango leaf extract for improving brain healthy situation. Total antioxidant capacity (T-AOC) in rat hippocampus was determined using T-AOC colorimetric assay kit (E-BC-K219-M).

Dilution of sample

It is recommended to take 2~3 samples with expected large difference to do pre-experiment before formal experiment and dilute the sample according to the result of the pre-experiment and the detection range (0.047-1.50 mmol/L).

The recommended dilution factor for different samples is as follows (for reference only):

Sample type

Dilution factor

10% Rat brain tissue homogenization

1

10% Rat liver tissue homogenization

1

10% Rat kidney tissue homogenization

1

10% Epipremnum aureum tissue homogenization

1

Human serum

1

Human saliva

1

Human urine

1

Rat serum

1

Note: When the sample was water soluble, the diluent is PBS (0.01 M, pH 7.4); 

When the sample is insoluble, the diluent is 80% ethanol.

Citations

  1. Foods (2023) IF: 5.561
    Treatment of Diabetes Nephropathy in Mice by Germinating Seeds of Euryale ferox through Improving Oxidative Stress

    DOI: 10.3390/foods12040767
    Sample: Serum
  2. Frontiers in Pharmacology (2021) IF: 5.811
    The Anti-Inflammatory Effect of a Combination of Five Compounds From Five Chinese Herbal Medicines Used in the Treatment of COPD

    DOI: 10.3389/fphar.2021.709702
    Sample: serum
  3. INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES (2021) IF: 5.924
    Comparison of Selenium Nanoparticles and Sodium Selenite on the Alleviation of Early Atherosclerosis by Inhibiting Endothelial Dysfunction and Inflammation in Apolipoprotein E-Deficient Mice

    DOI: 10.3390/ijms222111612

    PMID: 34769040

    		 Sample: serum
  4. Frontiers in Pharmacology (2022) IF: 5.988
    The effects of mango leaf extract during adolescence and adulthood in a rat model of schizophrenia

    DOI: 10.3389/fphar.2022.886514

    PMID: 35959428

    		 Sample: Brain tissue
  5. Drug Design Development and Therapy (2022) IF: 4.319
    The Preventive and Therapeutic Effects of Ajwa Date Fruit Extract Against Acute Diclofenac Toxicity-Induced Colopathy: An Experimental Study

    DOI: 10.2147/DDDT.S344247

    PMID: 35965961

    		 Sample: serum
  6. JOURNAL OF ETHNOPHARMACOLOGY (2021) IF: 4.36
    Suhuang antitussive capsule ameliorates post-infectious cough in mice through AhR-Nrf2 pathway

    DOI: 10.1016/j.jep.2021.114664

    PMID: 34555451

    		 Sample: lung tissue
  7. Scientific Reports (2021) IF: 4.38
    Effect of tiger milk mushroom (Lignosus rhinocerus) supplementation on respiratory health, immunity and antioxidant status: an open-label prospective study

    DOI: 10.1038/s41598-021-91256-6

    PMID: 34083710

    		 Sample: saliva
  8. BIOMEDICINE & PHARMACOTHERAPY (2020) IF: 4.545
    Marein ameliorates diabetic nephropathy by inhibiting renal sodium glucose transporter 2 and activating the AMPK signaling pathway in db/db mice and high glucose–treated HK-2 cells

    DOI: 10.1016/j.biopha.2020.110684
    Sample: serum
  9. Food Quality and Safety (2022) IF: 3.102
    Novel Functional Food from an invasive species Polygonum cuspidatum: Safety evaluation, Chemical Composition, and Hepatoprotective Effects

    DOI: 10.1093/fqsafe/fyac032
  10. Biomed Research International (2022) IF: 3.246
    Glycine Improved Cryopreserved Spermatozoa Quality in Achai Bull

    DOI: 10.1155/2022/8282387

    PMID: 35968237

    		 Sample: semen
  11. Frontiers in Veterinary Science (2022) IF: 3.412
    Ovarian Oxidative Stress Induced Follicle Depletion After Zona Pellucida 3 Vaccination Is Associated With Subfertility in BALB/c Mice

    DOI: 10.3389/fvets.2022.814827

    PMID: 35252419

    		 Sample: Ovarian
  12. PHARMACEUTICAL BIOLOGY (2022) IF: 3.503
    Combination of puerarin and tanshinone IIA alleviates ischaemic stroke injury in rats via activating the Nrf2/ARE signalling pathway

    DOI: 10.1080/13880209.2022.2070221

    PMID: 35635784

    		 Sample: brain tissue
  13. Journal of Inorganic and Organometallic Polymers and Materials (2023) IF: 3.518
    Preparation of Novel Composites of Polyvinyl Alcohol Containing Hesperidin Loaded ZnO Nanoparticles and Determination of Their Biological and Thermal Properties

    DOI: 10.1007/s10904-023-02532-z
  14. JOURNAL OF THE SCIENCE OF FOOD AND AGRICULTURE (2022) IF: 3.639
    Flaxseed oil ameliorates aging in D-galactose induced rats via altering gut microbiota and mitigating oxidative damage

    DOI: 10.1002/jsfa.12010

    PMID: 35567370

    		 Sample: serum,liver
  15. JOURNAL OF BIOMATERIALS SCIENCE-POLYMER EDITION (2022) IF: 3.682
    Synergistic effect of ZnO nanoparticles and hesperidin on the antibacterial properties of chitosan

    DOI: 10.1080/09205063.2022.2099668

    PMID: 35797143

    		 Sample: nanocomposites
  16. AMERICAN JOURNAL OF REPRODUCTIVE IMMUNOLOGY (2022) IF: 3.886
    Fetal hyperhomocysteinemia is associated with placental inflammation and early breakdown of maternal-fetal tolerance in Pre-term birth

    DOI: 10.1111/aji.13589

    PMID: 35750632

    		 Sample: serum
  17. International Journal of General Medicine (2023) IF: 2.145
    Hematological, Inflammatory, Coagulation, and Oxidative/Antioxidant Biomarkers as Predictors for Severity and Mortality in COVID-19: A Prospective Cohort-Study

    DOI: 10.2147/IJGM.S402206
    Sample: Serum
  18. Iranian Journal of Basic Medical Sciences (2020) IF: 2.146
    Magnesium supplementation enhances insulin sensitivity and decreases insulin resistance in diabetic rats

    DOI: 10.22038/ijbms.2020.40859.9650
    Sample: Serum
  19. Evidence-based Complementary and Alternative Medicine (2022) IF: 2.63
    Etlingera elatior Flower Aqueous Extract Protects against Oxidative Stress-Induced Nephropathy in a Rat Model of Type 2 Diabetes

    DOI: 10.1155/2022/2814196

    PMID: 35502173

    		 Sample: plasma
  20. WORK-A Journal of Prevention Assessment & Rehabilitation (2021) IF: 1.505
    Evaluation of oxidative stress and pro-inflammatory cytokines in occupationally cadmium exposed workers

    DOI: 10.3233/WOR-203302

    PMID: 33104053

    		 Sample: serum
  21. JOURNAL OF OCCUPATIONAL AND ENVIRONMENTAL MEDICINE (2020) IF: 1.642
    Spirometric Outcomes and Oxidative Stress Among Cement Factory Workers: Results From a Cross-Sectional Study

    DOI: 10.1097/JOM.0000000000001991

    PMID: 32796257

    		 Sample: serum
  22. Archives of Environmental & Occupational Health (2021) IF: 1.663
    Selenium, oxidative stress and inflammatory markers in handicraft workers occupationally exposed to lead

    DOI: 10.1080/19338244.2021.1968780

    PMID: 34423749

    		 Sample: serum
  23. Food Science & Nutrition (2019) IF: 1.747
    Coconut (Cocos nucifera L.) sap as a potential source of sugar: Antioxidant and nutritional properties

    DOI: 10.1002/fsn3.1191
    Sample: Coconut sap,sugar palm juice
  24. Livestock Science (2022) IF: 1.943
    Effects of nature identical essential oils (carvacrol, thymol and cinnamaldehyde) on growth performance of piglets and non-invasive markers of antioxidant status and calprotectin release

    DOI: 10.1016/j.livsci.2022.104959
    Sample: fecal
  25. Current Developments in Nutrition (2019)
    Yacon (Smallanthus sonchifolius): Effect on Integrity of Intestinal Barrier, Inflammatory Response and Oxidative Stress in Animal Model of Colon Cancer (P06-052-19)

    DOI: 10.1093/cdn/nzz031.P06-052-19
    Sample: Serum
  26. KEY ENGINEERING MATERIALS (2019)
    The Effect of Green Betle Leaf Gel (Piper Betle Leaf) to Total Antioxidant Capacity (TAC) Level after Scaling and Root Planing (SRP) Treatment

    DOI: 10.4028/www.scientific.net/KEM.829.220
    Sample: Gingival Crevicular Fluid
  27. International Journal of Food Science (2023)
    Fabrication of Nutraceutical Beverage from Saffron (Crocus sativus L.) Extract and Studying Its Health Effects

    DOI: 10.1155/2023/7130266
    Sample: serum
  28. Toxicology Reports (2019)
    Elevated reproductive toxicity effects of diclofenac after withdrawal: Investigation of the therapeutic role of melatonin

    DOI: 10.1016/j.toxrep.2019.06.009
    Sample: plasma
  29. International Journal of Reproductive Biomedicine (2021)
    Association between a marker of sperm DNA damage and sperm indices in infertile males in Benin City, Nigeria: A cross-sectional study

    DOI: 10.18502/ijrm.v19i2.8472
    Sample: Semen sample
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Reviews/Q&A

  • Show all
  • Reviews
  • Q&A
Q

M*****iSubmitted [ Aug 01 2022 ]

Asked: Why did it turn black when I added ABTS working solution?

Reply

A

adminSubmitted [ Aug 01 2022 ]

Answered: The reason may be that the ABTS working solution had been put away for too long after being prepared. It is recommended to prepare fresh working solution with needed amount before use.

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