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Total Antioxidant Capacity (T-AOC) Colorimetric Assay Kit (ABTS, Chemical Method)

Citations(21)

Cat.No.:E-BC-K271-M
Detection instrument: Microplate reader(730-740 nm)

Detection method: Colorimetric method

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Detection principle

The principle of the ABTS method for determining the T-AOC is as follows. ABTS is oxidized to green ABTS+ by appropriate oxidant, which can be inhibited if there exist antioxidants. The T-AOC of the sample can be determined and calculated by measuring the absorbance of ABTS+ at 734 nm. Trolox is an analog of VE and has a similar antioxidant capacity to that of VE. Trolox is used as a reference for other antioxidant antioxidants. For example, the T-AOC of Trolox is 1, then the antioxidant capacity of the other substance with the same concentration is showed by the ratio of its antioxidant capacity to Trolox antioxidant capacity.

Performance characteristics

Synonyms	T-AOC
Sample type	Serum,plasma,urine,saliva,tissue,cells
Sensitivity	0.05 mmol/L
Detection range	0.05-1.00 mmol/L
Detection method	Colorimetric method
Assay type	Quantitative
Assay time	20 min
Precision	Average inter-assay CV: 5%Average intra-assay CV: 4.100%
Other instruments required	Micropipettor, Centrifuge, Vortex mixer
Other reagents required	Double distilled water, 1 × PBS, 80% Ethanol
Storage	-20℃
Valid period	12 months

Dilution of sample

It is recommended to take 2~3 samples with expected large difference to do pre-experiment before formal experiment and dilute the sample according to the result of the pre-experiment and the detection range (0.05-1.00 mmol/L).

The recommended dilution factor for different samples is as follows (for reference only):

Sample type	Dilution factor
20% Tomato tissue homogenization	2-5
10% Mouse heart tissue homogenization	8-12
10% Mouse liver tissue homogenization	8-12
10% Mouse lung tissue homogenization	8-12
Human saliva	2-5
Human urine	15-30
Human serum	15-30
Human plasma	8-15

Note: The diluent is 1 × PBS or 80% ethanol.

Troubleshooting

Collection and treatment of common samples for metabolism assay detection

Questions and Answers of Metabolism Assay Kits

Citations

Frontiers in Pharmacology (2021) IF: 5.811

The Anti-Inflammatory Effect of a Combination of Five Compounds From Five Chinese Herbal Medicines Used in the Treatment of COPD

DOI: 10.3389/fphar.2021.709702
	Sample: Serum

INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES (2021) IF: 5.924

Comparison of Selenium Nanoparticles and Sodium Selenite on the Alleviation of Early Atherosclerosis by Inhibiting Endothelial Dysfunction and Inflammation in Apolipoprotein E-Deficient Mice

DOI: 10.3390/ijms222111612
PMID: 34769040	Sample: Serum

International Journal of Food Science (2023)

Fabrication of Nutraceutical Beverage from Saffron (Crocus sativus L.) Extract and Studying Its Health Effects

DOI: 10.1155/2023/7130266
	Sample: Serum

Toxicology Reports (2019)

Elevated reproductive toxicity effects of diclofenac after withdrawal: Investigation of the therapeutic role of melatonin

DOI: 10.1016/j.toxrep.2019.06.009
	Sample: Plasma

WORK-A Journal of Prevention Assessment & Rehabilitation (2021) IF: 1.505

Evaluation of oxidative stress and pro-inflammatory cytokines in occupationally cadmium exposed workers

DOI: 10.3233/WOR-203302
PMID: 33104053	Sample: Serum

JOURNAL OF OCCUPATIONAL AND ENVIRONMENTAL MEDICINE (2020) IF: 1.642

Spirometric Outcomes and Oxidative Stress Among Cement Factory Workers: Results From a Cross-Sectional Study

DOI: 10.1097/JOM.0000000000001991
PMID: 32796257	Sample: Serum

Archives of Environmental & Occupational Health (2021) IF: 1.663

Selenium, oxidative stress and inflammatory markers in handicraft workers occupationally exposed to lead

DOI: 10.1080/19338244.2021.1968780
PMID: 34423749	Sample: Serum

HIGH ALTITUDE MEDICINE & BIOLOGY (2022) IF: 1.981

A Nanocurcumin and Pyrroloquinoline Quinone Formulation Prevents Hypobaric Hypoxia-Induced Skeletal Muscle Atrophy by Modulating NF-κB Signaling Pathway

DOI: 10.1089/ham.2021.0127
PMID: 35384739	Sample: Gastrocnemius,Soleus Muscles

International Journal of General Medicine (2023) IF: 2.145

Hematological, Inflammatory, Coagulation, and Oxidative/Antioxidant Biomarkers as Predictors for Severity and Mortality in COVID-19: A Prospective Cohort-Study

DOI: 10.2147/IJGM.S402206
	Sample: Serum

Food Quality and Safety (2022) IF: 3.102

Novel Functional Food from an invasive species Polygonum cuspidatum: Safety evaluation, Chemical Composition, and Hepatoprotective Effects

DOI: 10.1093/fqsafe/fyac032

Biomed Research International (2022) IF: 3.246

Glycine Improved Cryopreserved Spermatozoa Quality in Achai Bull

DOI: 10.1155/2022/8282387
PMID: 35968237	Sample: Semen

Frontiers in Veterinary Science (2022) IF: 3.412

Ovarian Oxidative Stress Induced Follicle Depletion After Zona Pellucida 3 Vaccination Is Associated With Subfertility in BALB/c Mice

DOI: 10.3389/fvets.2022.814827
PMID: 35252419	Sample: Ovarian

PHARMACEUTICAL BIOLOGY (2022) IF: 3.503

Combination of puerarin and tanshinone IIA alleviates ischaemic stroke injury in rats via activating the Nrf2/ARE signalling pathway

DOI: 10.1080/13880209.2022.2070221
PMID: 35635784	Sample: Brain Tissue

Journal of Inorganic and Organometallic Polymers and Materials (2023) IF: 3.518

Preparation of Novel Composites of Polyvinyl Alcohol Containing Hesperidin Loaded ZnO Nanoparticles and Determination of Their Biological and Thermal Properties

DOI: 10.1007/s10904-023-02532-z

JOURNAL OF THE SCIENCE OF FOOD AND AGRICULTURE (2022) IF: 3.639

Flaxseed oil ameliorates aging in D-galactose induced rats via altering gut microbiota and mitigating oxidative damage

DOI: 10.1002/jsfa.12010
PMID: 35567370	Sample: Serum,Liver

JOURNAL OF BIOMATERIALS SCIENCE-POLYMER EDITION (2022) IF: 3.682

Synergistic effect of ZnO nanoparticles and hesperidin on the antibacterial properties of chitosan

DOI: 10.1080/09205063.2022.2099668
PMID: 35797143	Sample: Nanocomposites

Drug Design Development and Therapy (2022) IF: 4.319

The Preventive and Therapeutic Effects of Ajwa Date Fruit Extract Against Acute Diclofenac Toxicity-Induced Colopathy: An Experimental Study

DOI: 10.2147/DDDT.S344247
PMID: 35965961	Sample: Serum

JOURNAL OF ETHNOPHARMACOLOGY (2021) IF: 4.36

Suhuang antitussive capsule ameliorates post-infectious cough in mice through AhR-Nrf2 pathway

DOI: 10.1016/j.jep.2021.114664
PMID: 34555451	Sample: Lung Tissue

Scientific Reports (2021) IF: 4.38

Effect of tiger milk mushroom (Lignosus rhinocerus) supplementation on respiratory health, immunity and antioxidant status: an open-label prospective study

DOI: 10.1038/s41598-021-91256-6
PMID: 34083710	Sample: Saliva

BIOMEDICINE & PHARMACOTHERAPY (2020) IF: 4.545

Marein ameliorates diabetic nephropathy by inhibiting renal sodium glucose transporter 2 and activating the AMPK signaling pathway in db/db mice and high glucose–treated HK-2 cells

DOI: 10.1016/j.biopha.2020.110684
	Sample: Serum

Foods (2023) IF: 5.561

Treatment of Diabetes Nephropathy in Mice by Germinating Seeds of Euryale ferox through Improving Oxidative Stress

DOI: 10.3390/foods12040767
	Sample: Serum

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