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Total Antioxidant Capacity (T-AOC) Colorimetric Assay Kit (ABTS, Chemical Method)

Cat:E-BC-K271-M Citations (21)
Manual

Price: $ 500

Price: $ 180

Price: $ 120

Size:
500Assays 96T 48T
Quantity:
  • Sample Type: serum;plasma;urine;saliva;tissue;cells
  • Detection Instrument: Microplate reader(730-740 nm)
  • Reasearch Areas: Oxidative stress;Plant Physiology Series
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Product Details
Detection Principle The principle of the ABTS method for determining the T-AOC is as follows. ABTS is oxidized to green ABTS+ by appropriate oxidant, which can be inhibited if there exist antioxidants. The T-AOC of the sample can be determined and calculated by measuring the absorbance of ABTS+ at 734 nm. Trolox is an analog of VE and has a similar antioxidant capacity to that of VE. Trolox is used as a reference for other antioxidant antioxidants. For example, the T-AOC of Trolox is 1, then the antioxidant capacity of the other substance with the same concentration is showed by the ratio of its antioxidant capacity to Trolox antioxidant capacity.
Synonyms T-AOC
Sample Type serum,plasma,urine,saliva,tissue,cells
Sensitivity 0.05 mmol/L
Detection Range 0.05-1.00 mmol/L
Detection Method Colorimetric method
Assay Time 20 min
Precision inter-assay CV: 5% | intra-assay CV: 4.1%
Other Reagents Required Double distilled water, 1 × PBS, 80% Ethanol
Storage This product can be stored at -20°C for 12 months with shading light.
Valid Period 12 months
Dilution Of Sample

The recommended dilution factor for different samples is as follows (for reference only):

Sample type Dilution factor
20% Tomato tissue homogenization 2-5
10% Mouse heart tissue homogenization 8-12
10% Mouse liver tissue homogenization 8-12
10% Mouse lung tissue homogenization 8-12
Human saliva 2-5
Human urine 15-30
Human serum 15-30
Human plasma 8-15
The diluent is 1 × PBS or 80% ethanol. For the dilution of other sample types, please do pretest to confirm the dilution factor.