Current Position:Home>>Kits>>Metabolism Assays>> Total Cholesterol (TC) Colorimetric Assay Kit (Single Reagent, COD-PAP Method)
Cat.No.:E-BC-K109-S
Welcome to order from local distributors.
Protocols for metabolism assays
Add to cart Bulk requestFor research use only. Order now, ship in 3 days
Synonyms | TC |
Sample type | Serum,plasma,hydrothorax,animal tissue |
Sensitivity | 0.09 mmol/L |
Detection range | 0.09-25.85 mmol/L |
Detection method | Colorimetric method |
Assay type | Quantitative |
Assay time | 50 min |
Precision | Average inter-assay CV: 2.800%Average intra-assay CV: 1.100% |
Other instruments required | Micropipettor, Incubator, Centrifuge |
Other reagents required | Normal saline (0.9% NaCl), PBS (0.01 M, pH 7.4), Isopropanol |
Storage | 2-8℃ |
Valid period | 12 months |
It is recommended to take 2~3 samples with expected large difference to do pre-experiment before formal experiment and dilute the sample according to the result of the pre-experiment and the detection range (0.09-25.85 mmol/L).
The recommended dilution factor for different samples is as follows (for reference only):
Sample type
Dilution factor
Human serum
1
Mouse serum
1
Rat plasma
1
10% Mouse liver tissue
homogenate
1
10% Mouse kidney tissue
homogenate
1
10% Mouse heart tissue
homogenate
1
DOI: 10.1111/cpr.13332 |
|
Sample: liver |
PMID: 31356863 |
Sample: Serum |
Sample: serum |
Sample: Serum |
DOI: 10.1039/D2FO03509J |
|
Sample: serum |
PMID: 33784603 |
Sample: serum |
Sample: plasma |
PMID: 36229750 |
Sample: THP-1 cells |
Sample: serum |
Sample: serum,liver |
PMID: 36230124 |
Sample: liver,fecal,serum |
PMID: 35967043 |
Sample: serum |
PMID: 35151834 |
Sample: serum |
PMID: 34252510 |
Sample: serum |
Sample: blood |
PMID: 35429740 |
Sample: serum |
PMID: 31824338 |
Sample: Plasma |
Sample: serum |
PMID: 32488017 |
Sample: Plasma,Tissue homogenate |
PMID: 33060792 |
Sample: Plasma |
DOI: 10.1111/are.15474 |
|
Sample: serum |
Sample: plasma |
DOI: 10.1155/2020/1936406 |
|
PMID: 32117470 |
Sample: Plasma,Tissue homogenate |
Sample: liver tissue |
PMID: 31938118 |
Sample: serum |
DOI: 10.1111/are.15000 |
|
Sample: serum |
Sample: serum |
Sample: serum |
M*****iSubmitted [ Aug 02 2022 ]
Asked: I wonder if I need to measure the protein concentration for tissue sample?
Reply
adminSubmitted [ Aug 02 2022 ]
Answered: The homogenate medium is isopropanol, which has an effect on the determination of protein concentration, so it can be expressed directly by tissue fresh weight.