Total Cholesterol (TC) Colorimetric Assay Kit (Single Reagent, COD-PAP Method)

    • Biochemical Assay Kit-Elabscience
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    • Biochemical Assay Kit-Elabscience
    • Biochemical Assay Kit-Elabscience

      Catalog number:E-BC-K109-S

      Size:
      • 100Assays
      • 50Assays
      Qty:
      - +
      Price: $180
      Can detect 96 samples if without duplication

      Detection method: Colorimetric method

      Detection instrument: Spectrophotometer(510 nm)

      Lead Time: 7~10 daysWelcome to order from local distributors.

      Add to cart Compare Bulk request Manual

      Detection principle

      Total cholesterol includes free cholesterol and cholesterol esters. Cholesterol ester can be hydrolyzed by cholesterol esterase to produce cholesterol and free fatty acid. Cholesterol is oxidized by cholesterol oxidase to produce △4-cholestenone and hydrogen peroxide. In the presence of 4-aminoamylpyridine and phenol, hydrogen peroxide catalyze peroxidase to form red quinone compounds of benzoquinone imine phenizone.The color depth of the generated quinone is directly proportional to the cholesterol content. The absorbance values of the standard tube and the sample tube are measured respectively, and the cholesterol content in the sample can be calculated.

      Performance characteristics

      Synonyms TC
      Sample type serum,plasma,hydrothorax,animal tissue,cells
      Sensitivity 0.09 mmol/L
      Detection range 0.09-25.85 mmol/L
      Precision Average inter-assay CV: 2.8%Average intra-assay CV: 1.1%
      Other instruments required Micropipettor, Incubator, Centrifuge
      Other reagents required Normal saline (0.9% NaCl), PBS (0.01 M, pH 7.4), Isopropanol
      Storage 2-8℃
      Valid period 6 months

      Citations

      1. Alagebrium Mitigates Metabolic Insults in High Carbohydrate High Fat Diet Fed Rats
        IF: 5.574
        Journal: Pharmaceutical Sciences(2019)
        DOI: 99999999
        Products: E-BC-K109-S E-BC-K261-S 
        Sample type: Serum
        Reactivity: Rat
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      MSDS for ELISA ISO 9001

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