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Total Cholesterol (TC) Colorimetric Assay Kit (Single Reagent, COD-PAP Method)

  • Cat.No.:E-BC-K109-S

  • Detection instrument: Spectrophotometer(510 nm)

To Purchase E-BC-K109-S

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Detection principle

Total cholesterol includes free cholesterol and cholesterol esters. Cholesterol ester can be hydrolyzed by cholesterol esterase to produce cholesterol and free fatty acid. Cholesterol is oxidized by cholesterol oxidase to produce △4-cholestenone and hydrogen peroxide. In the presence of 4-aminoamylpyridine and phenol, hydrogen peroxide catalyze peroxidase to form red quinone compounds of benzoquinone imine phenizone.The color depth of the generated quinone is directly proportional to the cholesterol content. The absorbance values of the standard tube and the sample tube are measured respectively, and the cholesterol content in the sample can be calculated.

Performance characteristics

Synonyms TC
Sample type Serum,plasma,hydrothorax,animal tissue
Sensitivity 0.09 mmol/L
Detection range 0.09-25.85 mmol/L
Detection method Colorimetric method
Assay type Quantitative
Assay time 50 min
Precision Average inter-assay CV: 2.800%Average intra-assay CV: 1.100%
Other instruments required Micropipettor, Incubator, Centrifuge
Other reagents required Normal saline (0.9% NaCl), PBS (0.01 M, pH 7.4), Isopropanol
Storage 2-8℃
Valid period 12 months

Dilution of sample

It is recommended to take 2~3 samples with expected large difference to do pre-experiment before formal experiment and dilute the sample according to the result of the pre-experiment and the detection range (0.09-25.85 mmol/L).

The recommended dilution factor for different samples is as follows (for reference only):

Sample type

Dilution factor

Human serum

1

Mouse serum

1

Rat plasma

1

10% Mouse liver tissue homogenate

1

10% Mouse kidney tissue homogenate

1

10% Mouse heart tissue homogenate

1

Note:  The diluent of serum (plasma) is normal saline ( 0.9% NaCl ) or PBS ( 0.01 M, pH 7.4 ); The diluent of animal tissue  is anhydrous ethanol.

Citations

  1. CELL PROLIFERATION (2022) IF: 8.7550
    Hypothalamic Hnscr regulates glucose balance by mediating central inflammation and insulin signal

    DOI: 10.1111/cpr.13332

    Sample: liver
  2. PHARMACOLOGICAL RESEARCH (2019) IF: 7.658
    Dasatinib ameliorates chronic pancreatitis induced by caerulein via anti-fibrotic and anti-inflammatory mechanism

    DOI: 10.1016/j.phrs.2019.104357

    PMID: 31356863

    Sample: Serum
  3. mBio (2021) IF: 7.867
    Ileal Bile Acid Transporter Inhibitor Improves Hepatic Steatosis by Ameliorating Gut Microbiota Dysbiosis in NAFLD Model Mice | mBio

    DOI: 10.1128/mBio.01155-21

    Sample: serum
  4. Frontiers in Endocrinology (2022) IF: 6.055
    Total Flavonoids of Drynariae Rhizoma Improve Glucocorticoid-Induced Osteoporosis of Rats: UHPLC-MS-Based Qualitative Analysis, Network Pharmacology Strategy and Pharmacodynamic Validation

    DOI: 10.3389/fendo.2022.920931

    Sample: Serum
  5. Food & Function (2023) IF: 6.317
    Analysis of the mechanism of action of quercetin in the treatment of hyperlipidemia based on metabolomics and intestinal flora

    DOI: 10.1039/D2FO03509J

    Sample: serum
  6. EUROPEAN JOURNAL OF MEDICINAL CHEMISTRY (2021) IF: 6.514
    Design, synthesis, and biological evaluation of a novel dual peroxisome proliferator-activated receptor alpha/delta agonist for the treatment of diabetic kidney disease through anti-inflammatory mechanisms

    DOI: 10.1016/j.ejmech.2021.113388

    PMID: 33784603

    Sample: serum
  7. Frontiers in Cell and Developmental Biology (2021) IF: 6.684
    N6-Methyladenosine Methyltransferase METTL3 Promotes Angiogenesis and Atherosclerosis by Upregulating the JAK2/STAT3 Pathway via m6A Reader IGF2BP1.

    DOI: 10.3389/fcell.2021.731810

    Sample: plasma
  8. CELL BIOLOGY AND TOXICOLOGY (2022) IF: 6.819
    TRIM64 promotes ox-LDL-induced foam cell formation, pyroptosis, and inflammation in THP-1-derived macrophages by activating a feedback loop with NF-κB via IκBα ubiquitination

    DOI: 10.1007/s10565-022-09768-4

    PMID: 36229750

    Sample: THP-1 cells
  9. JOURNAL OF ETHNOPHARMACOLOGY (2022) IF: 5.195
    Integrating metabolomics and network pharmacology to investigate Panax japonicus prevents kidney injury in HFD/STZ-induced diabetic mice

    DOI: 10.1016/j.jep.2022.115893

    Sample: serum
  10. Food Bioscience (2022) IF: 5.318
    Ameliorative effects of monascin from red mold rice on alcoholic liver injury and intestinal microbiota dysbiosis in mice

    DOI: 10.1016/j.fbio.2022.102079

    Sample: serum,liver
  11. Foods (2022) IF: 5.561
    Monascuspiloin from Monascus-Fermented Red Mold Rice Alleviates Alcoholic Liver Injury and Modulates Intestinal Microbiota

    DOI: 10.3390/foods11193048

    PMID: 36230124

    Sample: liver,fecal,serum
  12. ACS Omega (2022) IF: 4.132
    Beneficial Effects of lncRNA-UC.360+ shRNA on Diabetic Cardiac Sympathetic Damage via NLRP3 Inflammasome-Induced Pyroptosis in Stellate Ganglion

    DOI: 10.1021/acsomega.2c03619

    PMID: 35967043

    Sample: serum
  13. JOURNAL OF ETHNOPHARMACOLOGY (2022) IF: 4.36
    Zexie Tang targeting FKBP38/mTOR/SREBPs pathway improves hyperlipidemia

    DOI: 10.1016/j.jep.2022.115101

    PMID: 35151834

    Sample: serum
  14. TOXICOLOGY LETTERS (2021) IF: 4.374
    Alpha-lipoic acid attenuates silica-induced pulmonary fibrosis by improving mitochondrial function via AMPK/PGC1α pathway activation in C57BL/6J mice

    DOI: 10.1016/j.toxlet.2021.07.003

    PMID: 34252510

    Sample: serum
  15. Journal of Functional Foods (2021) IF: 4.451
    Heracleum dissectum Ledeb. ethanol extract attenuates metabolic syndrome symptoms in high-fat diet-induced obese mice by activating adiponectin/AMPK signaling

    DOI: 10.1016/j.jff.2021.104581

    Sample: blood
  16. JOURNAL OF PSYCHIATRIC RESEARCH (2022) IF: 4.791
    Comparison of cognitive impairments with lipid profiles and inflammatory biomarkers in unipolar and bipolar depression

    DOI: 10.1016/j.jpsychires.2022.04.002

    PMID: 35429740

    Sample: serum
  17. Frontiers in Physiology (2019) IF: 3.201
    Dietary Bile Acids Enhance Growth, and Alleviate Hepatic Fibrosis Induced by a High Starch Diet via AKT/FOXO1 and cAMP/AMPK/SREBP1 Pathway in Micropterus salmoides

    DOI: 10.3389/fphys.2019.01430

    PMID: 31824338

    Sample: Plasma
  18. MEAT SCIENCE (2019) IF: 3.483
    Effects of naturally-produced lovastatin on carcass characteristics, muscle physico-chemical properties and lipid oxidation and cholesterol content in goats

    DOI: 10.1016/j.meatsci.2019.04.008

    Sample: serum
  19. Scientific Reports (2020) IF: 3.998
    Reproductive effects of subchronic exposure to acetamiprid in male rats

    DOI: 10.1038/s41598-020-65887-0

    PMID: 32488017

    Sample: Plasma,Tissue homogenate
  20. Scientific Reports (2020) IF: 3.998
    Disordered metabolism in mice lacking irisin

    DOI: 10.1038/s41598-020-74588-7

    PMID: 33060792

    Sample: Plasma
  21. AQUACULTURE RESEARCH (2021) IF: 2.082
    Quillaja saponaria and/or Yucca schidigera ameliorate water quality, growth performance, blood health, intestine and gills histomorphology of Nile tilapia, Oreochromis niloticus

    DOI: 10.1111/are.15474

    Sample: serum
  22. Journal of Developmental Origins of Health and Disease (2020) IF: 2.456
    Neonatal zingerone protects against the development of high-fructose diet-induced metabolic syndrome in adult Sprague-Dawley rats

    DOI: 10.1017/S2040174420000525

    Sample: plasma
  23. Cardiovascular Therapeutics (2020) IF: 2.538
    Bredemolic Acid Improves Cardiovascular Function and Attenuates Endothelial Dysfunction in Diet-Induced Prediabetes: Effects on Selected Markers

    DOI: 10.1155/2020/1936406

    PMID: 32117470

    Sample: Plasma,Tissue homogenate
  24. NUTRITION (2016) IF: 2.839
    Dietary sweet cherry anthocyanins attenuates diet-induced hepatic steatosis by improving hepatic lipid metabolism in mice

    DOI: 10.1016/j.nut.2016.01.007

    Sample: liver tissue
  25. International Journal of Clinical and Experimental Pathology (2018) IF: 1.396
    Pitavastatin attenuates atherosclerosis by suppressing NF-κB signaling in a high-cholesterol diet plus balloon catheter injury rabbit model

    PMID: 31938118

    Sample: serum
  26. AQUACULTURE RESEARCH (2020) IF: 1.748
    Dietary essential oil extract from sweet orange (Citrus sinensis) and bitter lemon (Citrus limon) peels improved Nile tilapia performance and health status

    DOI: 10.1111/are.15000

    Sample: serum
  27. Journal of Advanced Pharmaceutical Technology & Research (2020)
    Antihyperlipidemic effects of apple peel extract in high-fat diet-induced hyperlipidemic rats

    DOI: 10.4103/japtr.JAPTR_28_20

    Sample: serum
  28. Revista Brasileira de Medicina do Esporte (2022) IF: 0.652
    IMPACTS OF AEROBIC EXERCISE ON THE OBESITY OF ADOLESCENTS AND THEIR LIPID METABOLISM

    DOI: 10.1590/1517-8692202329012022_0163

    Sample: serum
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Reviews/Q&A

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  • Reviews
  • Q&A
Q

M*****iSubmitted [ Aug 02 2022 ]

Asked: I wonder if I need to measure the protein concentration for tissue sample?

Reply

A

adminSubmitted [ Aug 02 2022 ]

Answered: The homogenate medium is isopropanol, which has an effect on the determination of protein concentration, so it can be expressed directly by tissue fresh weight.

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