Current Position:Home>>Kits>>Metabolism Assays>> Total Glutathione (T-GSH)/Oxidized Glutathione (GSSG) Colorimetric Assay Kit
Cat.No.:E-BC-K097-M
Welcome to order from local distributors.
Protocols for metabolism assays
Add to cart Bulk requestFor research use only. Order now, ship in 3 days
Synonyms | T-GSH,GSSG |
Sample type | Serum,plasma,animal tissue,red blood cells,cultured cells |
Sensitivity | 0.36 μmol/L T-GSH |
Detection range | 0.36-30 μmol/L T-GSH |
Detection method | Colorimetric method |
Assay type | Quantitative |
Assay time | 100 min |
Precision | Average inter-assay CV: 3.900%Average intra-assay CV: 0.600% |
Other instruments required | Micropipettor, Centrifuge, Incubator, Vortex mixer |
Other reagents required | PBS (0.01 M, pH 7.4), Absolute ethanol |
Storage | -20℃ |
Valid period | 12 months |
The ratio of GSH/GSSG was significantly decreased in CuO2 treated groups.
XX Li et al developed a novel nanoreactor activated by tumor microenvironment for chemiluminescence imaging, can be used for tumor resistance. The ratio between total glutathione and oxidized glutathione (GSH/GSSG) in human MCF-7 cell was determined using GSH/GSSG colorimetric assay kit (E-BC-K097-M).
It is recommended to take 2~3 samples with expected large difference to do pre-experiment before formal experiment and dilute the sample according to the result of the pre-experiment and the detection range (0.36-30 μmol/L T-GSH).
The recommended dilution factor for different samples is as follows (for reference only):
Sample type |
Dilution factor |
Human plasma |
1 |
10% Rat liver tissue homogenate |
10-20 |
10% Rat kidney tissue homogenate |
1 |
10% Rat heart tissue homogenate |
10-20 |
10% Mouse brain tissue homogenate |
2-5 |
HepG2 cells |
1 |
Sample: MCF-7 cells |
Sample: lung tissue,alveolar epithelial Type2 cells (AEC2) |
PMID: 36358477 |
Sample: liver,serum |
PMID: 35936786 |
Sample: mammary adenocarcinoma tissue |
PMID: 32768570 |
Sample: Tissue homogenate |
PMID: 36069237 |
Sample: colon tissue |
PMID: 35597951 |
Sample: testis tissue |
DOI: 10.1039/D1FO03577K |
|
PMID: 34994761 |
Sample: renal |
Sample: colon |
PMID: 35066100 |
Sample: breast tumor tissue |
PMID: 35220872 |
PMID: 35635784 |
Sample: brain tissue |
Sample: blood |
DOI: 10.1002/jsfa.11611 |
|
PMID: 34708426 |
Sample: renal cortex |
PMID: 32142764 |
Sample: Tissue homogenate |
DOI: 10.1111/jfbc.14306 |
|
PMID: 35766031 |
Sample: colon tissue,brain tissue |
Sample: HaCaT cell |
Sample: hippocampi |
PMID: 33165681 |
Sample: Tissue homogenate |
Sample: brains |
PMID: 36070970 |
Sample: sciatic nerve |
DOI: 10.1111/jfbc.13994 |
|
PMID: 34778972 |
Sample: brain |
DOI: 10.3390/ani11020399 |
|
PMID: 33557433 |
Sample: Plasma |
DOI: 10.1155/2020/5369797 |
|
PMID: 32328233 |
Sample: Tissue homogenate |
M*****aSubmitted [ Sep 23 2022 ]
Asked: What is GSSG homogenate medium and diluent?
Reply
adminSubmitted [ Sep 23 2022 ]
Answered: The homogenate medium and diluent are all reagent 3 working solution.
M*****aSubmitted [ Sep 06 2022 ]
Asked: Human Oxidized Glutathione ELISA Kit, 96 T, (E-BC-K097-M), does BCA need to be used?
Reply
adminSubmitted [ Sep 06 2022 ]
Answered: There is no need to use BCA kit, no matter what the sample is.