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Cat.No.:E-BC-K097-S
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Synonyms | T-GSH,GSSG |
Sample type | Serum,plasma,animal tissue,red blood cells,cultured cells |
Sensitivity | 0.12 μmol/L T-GSH |
Detection range | 0.12-30 μmol/L T-GSH |
Detection method | Colorimetric method |
Assay type | Quantitative |
Assay time | 180 min |
Precision | Average inter-assay CV: 4.700%Average intra-assay CV: 0.900% |
Other instruments required | Micropipettor, Incubator, Vortex mixer, Centrifuge |
Other reagents required | PBS(0.01 M, pH 7.4), Absolute ethanol |
Storage | Reagent 2, Reagent 3, Reagent 4, Reagent 8, Reagent 9: -20℃; others: 2-8℃ |
Valid period | 12 months |
It is recommended to take 2~3 samples with expected large difference to do pre-experiment before formal experiment and dilute the sample according to the result of the pre-experiment and the detection range (0.12-30 μmol/L T-GSH).
The recommended dilution factor for different samples is as follows (for reference only):
Sample type |
Dilution factor |
Human serum |
1 |
Rat plasma |
1 |
Mouse serum |
1 |
10% Rat heart tissue homogenate |
10 |
10% Rat liver tissue homogenate |
60 |
10% Rat brain tissue homogenate |
10 |
Note: The diluent is reagent 3.
PMID: 33706136 |
Sample: Cell lysate |
DOI: 10.1039/D1FO03577K |
|
PMID: 34994761 |
Sample: renal |
PMID: 33432709 |
Sample: Cell sample |
PMID: 35220872 |
PMID: 35635784 |
Sample: brain tissue |
Sample: blood |
DOI: 10.1002/jsfa.11611 |
|
PMID: 34708426 |
Sample: renal cortex |