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Zinc (Zn) Colorimetric Assay Kit

Cat.No.:E-BC-K137-M
Detection instrument: Microplate reader(545-575 nm)

Detection method: Colorimetric method

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48T

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Protocols for metabolism assays

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Detection principle

The zinc ion in the sample react with 5-Br-PADAP to produce the colored complex. The depth of color is directly proportional to the concentration of zinc ion. Zinc ion content can be calculated by measuring the OD values at 560 nm.

Performance characteristics

Synonyms	Zn
Sample type	Serum,plasma,urine,milk
Sensitivity	0.418 μmol/L
Detection range	0.748 -46.2 μmol/L
Detection method	Colorimetric method
Assay type	Quantitative
Assay time	20 min
Precision	Average inter-assay CV: 4%Average intra-assay CV: 2.700%
Other instruments required	Micropipettor, Vortex mixer
Other reagents required	Deionized water
Storage	2-8℃
Valid period	12 months

Dilution of sample

It is recommended to take 2~3 samples with expected large difference to do pre-experiment before formal experiment and dilute the sample according to the result of the pre-experiment and the detection range (0.748 -46.2 μmol/L).

The recommended dilution factor for different samples is as follows (for reference only):

Sample type	Dilution factor
Human urine	1
Human serum	1
Human milk	1
Rat serum	1

Note: The diluent is deionized water.

Troubleshooting

Collection and treatment of common samples for metabolism assay detection

Questions and Answers of Metabolism Assay Kits

Citations

Frontiers in Neuroscience (2021) IF: 4.677
Imbalances in Copper or Zinc Concentrations Trigger Further Trace Metal Dyshomeostasis in Amyloid-Beta Producing Caenorhabditis elegans

DOI: 10.3389/fnins.2021.755475

PMID: 34707479

Reviews/Q&A

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Reviews
Q&A

M**********dSubmitted [ Aug 26 2022 ]

Asked: Can I measure the zinc concentration in cells?

adminSubmitted [ Aug 26 2022 ]

Answered: It's no avaliable at present, as the concentration in cells is too low to be measured.

D********dSubmitted [ Aug 04 2022 ]

Asked: Can I use this kit for tissue sample? You didn\'t mention that in the manual.

adminSubmitted [ Aug 04 2022 ]

Answered: Yes, the sample has been verified. Thanks for your question, and we'll improve our manual as soon as possible.

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