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QuicKey ELISA Kits

QuicKey ELISA Kit is an improvement of the traditional sandwich ELISA Kit. It simplifies the operation process, and only 2 washing steps and 3 incubation steps are required. It shortens the experiment time from 3.5 hours to 2.5 hours without compromising high sensitivity, specificity, accuracy and stability. The QuicKey ELISA Kit guarantees the experiment performance and greatly improve the detection efficiency!

>> QuicKey ELISA Kits for Nephropathy

	Traditional ELISA	QuicKey ELISA	What it means for you
Assay Time	3.5h	2.5h	Saving 1h
Assay Procedure	7 steps	6 steps	Remove the calculation and dilution process of Biotinylated Detection Ab, add it directly
Storage	Different components should be separately stored at -20°C or 4°C	All components are stored at 4°C	Same storage condition, avoid affecting the validity period due to improper storage
Difference Between Biotinylated Detection Ab and Concentrated HRP Conjugate	Both are concentrated, distinguished by the color of the cap, the working solutions are colorless	Biotinylated Detection Ab is colorless, HRP Conjugate working solution is red	Less risk of mistakes

How Does Elabscience^® QuicKey ELISA Work?

QuicKey ELISA

	1. Add 50μL standard or sample to the wells, immediately add 50μL Biotinylated Detection Ab working solution to each well. Incubate for 90 min at 37°C
	2. Aspirate and wash the plate for 3 times
	3. Add 100μL HRP conjugate working solution. Incubate for 30 min at 37°C. Aspirate and wash the plate for 5 times
	4. Add 90μL Substrate Reagent. Incubate for 15 min at 37°C
	5. Add 50μL Stop Solution
	6. Read the plate at 450nm immediately. Calculation of the results

Traditional Sandwich ELISA

	1. Add 100μL standard or sample to the wells. Incubate for 90 min at 37°C
	2. Discard the liquid, immediately add 100μL Biotinylated Detection Ab working solution to each well. Incubate for 60 min at 37°C
	3. Aspirate and wash the plate for 3 times
	4. Add 100μL HRP conjugate working solution. Incubate for 30 min at 37°C. Aspirate and wash the plate for 5 times
	5. Add 90μL Substrate Reagent. Incubate for 15 min at 37°C
	6. Add 50μL Stop Solution
	7. Read the plate at 450nm immediately. Calculation of the results