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Caspase 8 Activity Colorimetric Assay Kit

  • Cat.No.:E-CK-A312

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Detection principle

Caspase (Cysteine-requiring Aspartate Protease) is a protease family that plays an important role in the process of apoptosis. Caspase 8 (also known as FLICE, MACH or mch5) is considered to be a caspase in the upstream of apoptotic signal transduction. Caspase 8 is activated in Fas-receptor and TNFR-1-mediated apoptotic process, forming a dimer composed of P18 and p10, which further activates downstream Caspase 4, Caspase 6, Caspase 9 and Caspase 10. Caspase 8 exists in the form of prozyme in the normal state and has no activity. However, Caspase 8 is activated in the apoptotic stage and participates in the apoptotic process. This kit is used to conjugate Caspase 8 sequence-specific peptides acetyl-Ile-Glu-Val-Asp p-nitroanilide (Ac-IETD-pNA) to yellow group p-nitroaniline (pNA). When the substrate is cut by Caspase 8, the yellow group pNA is dissociated. pNA has an absorption peak at 405 nm. Measure the OD value at 405 nm and then Caspase 8 activity can be calculated accordingly.

General Information

Application Caspase
Assay type Qualitative
Detection method Colorimetric Method
Sample type Cell and tissue samples
Assay time 4-6 hours
Detection instrument Spectrophotometer
Other reagents required PBS(E-BC-R187),Bradford Protein Determination Kit(E-BC-K168-S or E-BC-K168-M)
Storage -20°C
Shipping Ice bag
Expiration date 12 months

Citations

From now on, if you have published a paper by using any of our products since 1/1/2019, fill out the “Elabscience Publication Reward Application Form”carefully and send it to orders@elabscience.com, we will get back to you with the reward after we confirm it ASAP!

View more details about our Publication Reward >>

  1. Molecular and Cellular Endocrinology (2020) IF: 3.871
    Poly(ADP-ribose) polymerase-2 is essential for endometrial receptivity and blastocyst implantation, and regulated by caspase-8

    DOI: 10.1016/j.mce.2020.110946

    PMID: 32679243

    Sample: Tissue homogenate

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