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Cat.No.:E-BC-K125-S
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Synonyms | ChAT |
Sample type | Animal tissue |
Sensitivity | 1.21 U/g fresh weigh |
Detection range | 1.21-40 U/g fresh we |
Detection method | Colorimetric method |
Assay type | Enzyme Activity |
Assay time | 80 min |
Precision | Average inter-assay CV: 9.200%Average intra-assay CV: 5% |
Other instruments required | Micropipettor, Centrifuge, Incubator, Water bath, Vortex mixer |
Other reagents required | Normal saline (0.9% NaCl), PBS (0.01 M, pH 7.4) |
Storage | Reagent 2, Reagent 3, Reagent 6: -20℃, others: 2-8℃ |
Valid period | 12 months |
It is recommended to take 2~3 samples with expected large difference to do pre-experiment before formal experiment and dilute the sample according to the result of the pre-experiment and the detection range (1.21-40 U/g fresh weight).
The recommended dilution factor for different samples is as follows (for reference only):
Sample type |
Dilution factor |
20% Mouse brain tissue homogenate |
1-2 |
20% Rat heart tissue homogenate |
1 |
20% Rat liver tissue homogenate |
1 |
20% Mouse kidney tissue homogenate |
1 |
20% Rat lung tissue homogenate |
1 |
Sample: Brain |
DOI: 10.3390/pr11010159 |
|
Sample: Hippocampal |
PMID: 35895153 |
Sample: Hippocampus Tissue |
PMID: 32679768 |
Sample: Tissue Homogenate |
PMID: 34073796 |
Sample: Tissue Homogenate |
R***iSubmitted [ Apr 08 2024 ]
Asked: E-BC-K125-S Can this kit be used for estimation of choline in the serum samples also?
Reply
adminSubmitted [ Apr 08 2024 ]
Answered: We'll reply you by email soon.
P***********lSubmitted [ Jul 14 2023 ]
Asked: what is the preheating temperature for working solution that you had mentioned in the procedure?
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adminSubmitted [ Jul 14 2023 ]
Answered: Hello Parvati,thanks for your support.The preheating temperature for working solution that you had mentioned in the procedure is 37℃.
M**********dSubmitted [ Aug 25 2022 ]
Asked: Is the kit suitable for cell?
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adminSubmitted [ Aug 25 2022 ]
Answered: Yes, it takes about 3*10^6 cells with 200 micro-liter of saline or PBS based on the cell types.
D********dSubmitted [ Aug 03 2022 ]
Asked: Why can\'t I see any color development after the whole process?
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adminSubmitted [ Aug 03 2022 ]
Answered: The detection wavelength is 324 nm which is in the range of ultra-violet, thus people cannot see it but the absorbance can be detected by the instrument.
b***lSubmitted [ May 23 2019 ]
Asked: how can i prepare Boiled sample?
Reply