Choline Acetyltransferase (ChAT) Activity Assay Kit (Tissue Samples)

    • Biochemical Assay Kit-Elabscience
    • Biochemical Assay Kit-Elabscience
    • Biochemical Assay Kit-Elabscience

      Catalog number:E-BC-K125-S

      • 100Assays
      • 50Assays
      - +
      Price: $280
      Can detect 50 samples if without duplication

      Detection method: Colorimetric method

      Detection instrument: Spectrophotometer(324 nm)

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      Detection principle

      Acetyl-CoA can react with choline under the catalysis of choline acetyltransferase (ChAT) to produce coenzyme A (CoA), CoA can combine with the 4, 4-dithiopyridine. The activity of ChAT can be calculated indirectly by measuring the OD value at 324 nm.

      Performance characteristics

      Synonyms ChAT
      Sample type Animal tissue
      Sensitivity 1.21 U/g fresh weigh
      Detection range 1.21-40 U/g fresh we
      Detection method Colorimetric method
      Assay type Enzyme Activity
      Assay time 80 min
      Precision Average inter-assay CV: 9.2%Average intra-assay CV: 5.0%
      Other instruments required Micropipettor, Centrifuge, Incubator, Water bath, Vortex mixer
      Other reagents required Normal saline (0.9% NaCl), PBS (0.01 M, pH 7.4)
      Storage Reagent 2, Reagent 3, Reagent 6: -20℃, others: 2-8℃
      Valid period 3 months

      Dilution of sample

      It is recommended to take 2~3 samples with expected large difference to do pre-experiment before formal experiment and dilute the sample according to the result of the pre-experiment and the detection range (1.21-40 U/g fresh weight).

      The recommended dilution factor for different samples is as follows (for reference only):

      Sample type

      Dilution factor

      20% Mouse brain tissue homogenate


      20% Rat heart tissue homogenate


      20% Rat liver tissue homogenate


      20% Mouse kidney tissue homogenate


      20% Rat lung tissue homogenate


      Note: The diluent is normal saline (0.9% NaCl) or PBS (0.01 M, pH 7.4).


      1. Enteromorpha prolifera Extract Improves Memory in Scopolamine-Treated Mice via Downregulating Amyloid-β Expression and Upregulating BDNF/TrkB Pathway
        IF: 5.014
        Journal: Antioxidants(2020)
        DOI: 10.3390/antiox9070620
        PMID: 32679768
        Products: E-BC-K125-S 
        Sample type: Tissue homogenate
        Reactivity: Mouse
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      b***lSubmitted [ May 23 2019 ]

      Asked: how can i prepare Boiled sample?


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