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Glycerol Colorimetric Assay Kit

Cat.No.:E-BC-K340-M
Detection instrument: Microplate reader (500-520 nm, optimum wavelength: 510 nm)

Detection method: Colorimetric method

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48T

Price: $280

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Detection principle

Glycerol is transformed by enzyme to produce hydrogen peroxide, which is catalyzed by oxidase in the presence of 4-amino-antipyrprid and phenol to produce red quinones, the color of which is proportional to the content of glycerol.

Performance characteristics

Sample type	serum (plasma), animal tissue, cell
Sensitivity	0.01mmol/L
Detection range	0.01-1.00 mmol/L
Detection method	Colorimetric method
Assay type	Quantitative
Assay time	10 min
Precision	Average inter-assay CV: 8%Average intra-assay CV: 4%
Other instruments required	Water bath
Other reagents required	Double distilled water, Isopropanol (AR), PBS(0.01 M，pH 7.4)
Storage	2-8℃
Valid period	12 months

Troubleshooting

Collection and treatment of common samples for metabolism assay detection

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