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Human CTXⅠ(Cross Linked C-telopeptide of Type Ⅰ Collagen) ELISA Kit

  • Cat.No.:E-EL-H0835

  • Reactivity: Human

To Purchase E-EL-H0835

Size:
  • 96T
  • 48T
  • 24T
  • 96T*5
  • 96T*10
Price: $609
Qty:

Product Details

Properties

Assay type Sandwich-ELISA
Format 96T/48T
Assay time 3.5h
Detection range 0.16-10 ng/mL
Sensitivity 0.10 ng/mL
Sample type &Sample volume serum, plasma and other biological fluids; 100μL
Specificity This kit recognizes Human CTXⅠ in samples. No significant cross-reactivity or interference between Human CTXⅠ and analogues was observed.
Reproducibility Both intra-CV and inter-CV are < 10%.
Application This ELISA kit applies to the in vitro quantitative determination of Human CTXⅠ concentrations in serum, plasma and other biological fluids.

Test Principle

This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Human CTXⅠ. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human CTXⅠ and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human CTXⅠ, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Human CTXⅠ. You can calculate the concentration of Human CTXⅠ in the samples by comparing the OD of the samples to the standard curve.

Kit components & Storage

An unopened kit can be stored at 2-8℃ for 1 month. If the kit is not supposed to be used within 1 month, store the components separately according to the following conditions once the kit is received.
ItemSpecificationsStorage
Micro ELISA Plate(Dismountable) 96T: 8 wells ×12 strips
48T: 8 wells ×6 strips
-20℃, 6 months
Reference Standard 96T: 2 vials
48T: 1 vial
Concentrated Biotinylated Detection Ab (100×) 96T: 1 vial, 120 μL
48T: 1 vial, 60 μL
Concentrated HRP Conjugate (100×) 96T: 1 vial, 120 μL
48T: 1 vial, 60 μL
-20℃(Protect from light), 6 months
Reference Standard & Sample Diluent 1 vial, 20 mL 2-8°C, 6 months
Biotinylated Detection Ab Diluent 1 vial, 14 mL
HRP Conjugate Diluent 1 vial, 14 mL
Concentrated Wash Buffer (25×) 1 vial, 30 mL
Substrate Reagent 1 vial, 10 mL 2-8℃(Protect from light)
Stop Solution 1 vial, 10 mL 2-8°C
Plate Sealer 5 pieces
Manual 1 copy
Certificate of Analysis 1 copy

Technical Data

Precision

Intra-assay Precision (Precision within an assay): 3 samples with low, mid range and high level Human CTXⅠ were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, mid range and high level Human CTXⅠ were tested on 3 different plates, 20 replicates in each plate.

  Intra-assay Precision Inter-assay Precision
Sample 1 2 3 1 2 3
n 20 20 20 20 20 20
Mean (ng/mL) 0.52 1.08 4.39 0.49 0.99 4.46
Standard deviation 0.04 0.06 0.23 0.03 0.05 0.21
CV (%) 7.69 5.56 5.24 6.12 5.05 4.71

Recovery

The recovery of Human CTXⅠ spiked at three different levels in samples throughout the range of the assay was evaluated in various matrices.

Sample Type Range (%) Average Recovery (%)
Serum(n=8) 88-101 93
EDTA plasma (n=8) 94-109 100
Cell culture media (n=8) 88-100 93

Linearity

Samples were spiked with high concentrations of Human CTXⅠ and diluted with Reference Standard & Sample Diluent to produce samples with values within the range of the assay.

Target Information

SynonymsCTX-I, CTX1
Research AreaMetabolism, Cardiovascular, Cancer

Assay Procedures

elisa assay procedure 1

1. Add 100μL standard or sample to the wells. Incubate for 90 min at 37°C

elisa assay procedure 2

2. Discard the liquid, immediately add 100μL Biotinylated Detection Ab working solution to each well. Incubate for 60 min at 37°C

elisa assay procedure 3

3. Aspirate and wash the plate for 3 times

elisa assay procedure 4

4. Add 100μL HRP conjugate working solution. Incubate for 30 min at 37°C. Aspirate and wash the plate for 5 times

elisa assay procedure 5

5. Add 90μL Substrate Reagent. Incubate for 15 min at 37°C

elisa assay procedure 6

6. Add 50μL Stop Solution

elisa assay procedure 7

7. Read the plate at 450nm immediately. Calculation of the results

Citations

From now on, if you have published a paper by using any of our products since 1/1/2019, fill out the “Elabscience Publication Reward Application Form”carefully and send it to orders@elabscience.com, we will get back to you with the reward after we confirm it ASAP!

View more details about our Publication Reward >>

  1. JOURNAL OF NUTRITIONAL BIOCHEMISTRY (2022) IF: 6.117
    Maternal omega-3 LC-PUFA supplementation programs an improved bone mass in the offspring with a more pronounced effect in females than males at adulthood

    DOI: 10.1016/j.jnutbio.2022.109245

    Sample: plasma
  2. Biomolecules (2020) IF: 4.082
    Salivary Osteocalcin as Potential Diagnostic Marker of Periodontal Bone Destruction among Smokers

    DOI: 10.3390/biom10030380

    Sample: saliva
  3. Human Cell (2022) IF: 4.374
    METTL14 upregulates TCF1 through m6A mRNA methylation to stimulate osteogenic activity in osteoporosis

    DOI: 10.1007/s13577-022-00825-y

    Sample: serum
  4. OSTEOARTHRITIS AND CARTILAGE (2017) IF: 4.742
    Associations between serum ghrelin and knee symptoms, joint structures and cartilage or bone biomarkers in patients with knee osteoarthritis

    DOI: 10.1016/j.joca.2017.05.015

    Sample: Serum
  5. CALCIFIED TISSUE INTERNATIONAL (2019) IF: 3.265
    Effects of High-Intensity Interval Running Versus Cycling on Sclerostin, and Markers of Bone Turnover and Oxidative Stress in Young Men

    DOI: 10.1007/s00223-019-00524-1

    PMID: 30671591

    Sample: Serum
  6. Biomed Research International (2020) IF: 2.276
    Osteokines and Bone Markers at Rest and following Plyometric Exercise in Pre- and Postmenopausal Women

    DOI: 10.1155/2020/7917309

    PMID: 33145358

    Sample: Serum
  7. CLINICAL RHEUMATOLOGY (2019) IF: 2.293
    Associations between serum IL-8 and knee symptoms, joint structures, and cartilage or bone biomarkers in patients with knee osteoarthritis

    DOI: 10.1007/s10067-019-04718-8

    PMID: 31377918

    Sample: Serum
  8. Biomed Research International (2018) IF: 2.583
    Response of Sclerostin and Bone Turnover Markers to High Intensity Interval Exercise in Young Women: Does Impact Matter?

    DOI: 10.1155/2018/4864952

    Sample: serum
  9. Archives of Osteoporosis (2021) IF: 2.617
    Age-related trends and reference intervals of cross-linked C-telopeptide of type I collagen and procollagen type I N-propeptide from a reference population of Sri Lankan adult women

    DOI: 10.1007/s11657-021-01022-4

    PMID: 34727246

    Sample: serum
  10. Children-Basel (2021) IF: 2.863
    Circulating Levels of Bone Markers after Short-Term Intense Training with Increased Dairy Consumption in Adolescent Female Athletes

    DOI: 10.3390/children8110961

    Sample: serum
  11. ANGLE ORTHODONTIST (2019) IF: 1.88
    Comparison of BALP, CTX-I, and IL-4 levels around miniscrew implants during orthodontic tooth movement between two different amounts of force

    DOI: 10.2319/071718-520.1

    PMID: 30730198

    Sample: Gingival Crevicular Fluid
  12. Journal of Dental Sciences (2019) IF: 0.798
    Diagnostic accuracy of salivary biomarkers of bone turnover in identifying patients with periodontitis in a Saudi Arabian population

    DOI: 10.1016/j.jds.2019.03.002

    PMID: 31534637

    Sample: Saliva
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