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Human CTXⅠ(Cross Linked C-telopeptide of Type Ⅰ Collagen) ELISA Kit

  • Cat.No.:E-EL-H0835

  • Reactivity: Human

To Purchase E-EL-H0835

Size:
  • 96T
  • 48T
  • 24T
  • 96T*5
  • 96T*10
Price: $609
Qty:

Product Details

Properties

Assay type Sandwich-ELISA
Format 96T/48T
Assay time 3.5h
Detection range 0.16-10 ng/mL
Sensitivity 0.10 ng/mL
Sample type &Sample volume serum, plasma and other biological fluids; 100μL
Specificity This kit recognizes Human CTXⅠ in samples. No significant cross-reactivity or interference between Human CTXⅠ and analogues was observed.
Reproducibility Both intra-CV and inter-CV are < 10%.
Application This ELISA kit applies to the in vitro quantitative determination of Human CTXⅠ concentrations in serum, plasma and other biological fluids.

Test Principle

This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Human CTXⅠ. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human CTXⅠ and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human CTXⅠ, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Human CTXⅠ. You can calculate the concentration of Human CTXⅠ in the samples by comparing the OD of the samples to the standard curve.

Kit components & Storage

An unopened kit can be stored at 2-8℃ for 1 month. If the kit is not supposed to be used within 1 month, store the components separately according to the following conditions once the kit is received.
ItemSpecificationsStorage
Micro ELISA Plate(Dismountable) 96T: 8 wells ×12 strips
48T: 8 wells ×6 strips
-20℃, 6 months
Reference Standard 96T: 2 vials
48T: 1 vial
Concentrated Biotinylated Detection Ab (100×) 96T: 1 vial, 120 μL
48T: 1 vial, 60 μL
Concentrated HRP Conjugate (100×) 96T: 1 vial, 120 μL
48T: 1 vial, 60 μL
-20℃(Protect from light), 6 months
Reference Standard & Sample Diluent 1 vial, 20 mL 2-8°C, 6 months
Biotinylated Detection Ab Diluent 1 vial, 14 mL
HRP Conjugate Diluent 1 vial, 14 mL
Concentrated Wash Buffer (25×) 1 vial, 30 mL
Substrate Reagent 1 vial, 10 mL 2-8℃(Protect from light)
Stop Solution 1 vial, 10 mL 2-8°C
Plate Sealer 5 pieces
Manual 1 copy
Certificate of Analysis 1 copy

Technical Data

Precision

Intra-assay Precision (Precision within an assay): 3 samples with low, mid range and high level Human CTXⅠ were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, mid range and high level Human CTXⅠ were tested on 3 different plates, 20 replicates in each plate.

  Intra-assay Precision Inter-assay Precision
Sample 1 2 3 1 2 3
n 20 20 20 20 20 20
Mean (ng/mL) 0.52 1.08 4.39 0.49 0.99 4.46
Standard deviation 0.04 0.06 0.23 0.03 0.05 0.21
CV (%) 7.69 5.56 5.24 6.12 5.05 4.71

Recovery

The recovery of Human CTXⅠ spiked at three different levels in samples throughout the range of the assay was evaluated in various matrices.

Sample Type Range (%) Average Recovery (%)
Serum(n=8) 88-101 93
EDTA plasma (n=8) 94-109 100
Cell culture media (n=8) 88-100 93

Linearity

Samples were spiked with high concentrations of Human CTXⅠ and diluted with Reference Standard & Sample Diluent to produce samples with values within the range of the assay.

Target Information

SynonymsCTX-I, CTX1, CTXI

Assay Procedures

elisa assay procedure 1

1. Add 100μL standard or sample to the wells. Incubate for 90 min at 37°C

elisa assay procedure 2

2. Discard the liquid, immediately add 100μL Biotinylated Detection Ab working solution to each well. Incubate for 60 min at 37°C

elisa assay procedure 3

3. Aspirate and wash the plate for 3 times

elisa assay procedure 4

4. Add 100μL HRP conjugate working solution. Incubate for 30 min at 37°C. Aspirate and wash the plate for 5 times

elisa assay procedure 5

5. Add 90μL Substrate Reagent. Incubate for 15 min at 37°C

elisa assay procedure 6

6. Add 50μL Stop Solution

elisa assay procedure 7

7. Read the plate at 450nm immediately. Calculation of the results

Citations

From now on, if you have published a paper by using any of our products since 1/1/2019, fill out the “Elabscience Publication Reward Application Form”carefully and send it to orders@elabscience.com, we will get back to you with the reward after we confirm it ASAP!

View more details about our Publication Reward >>

  1. Osteoarthritis and Cartilage (2017) IF: 4.742
    Associations Between Serum Ghrelin And Knee Symptoms, Joint Structures And Cartilage Or Bone Biomarkers In Patients With Knee Osteoarthritis.

    DOI: 10.1016/j.joca.2017.05.015

    PMID: 28602782

    Sample: Serum
  2. Calcified tissue international (2019) IF: 3.293
    Effects of High-Intensity Interval Running Versus Cycling on Sclerostin, and Markers of Bone Turnover and Oxidative Stress in Young Men

    DOI: 10.1007/s00223-019-00524-1

    PMID: 30671591

    Sample: Serum
  3. Clinical Rheumatology (2019) IF: 2.394
    Associations between serum IL-8 and knee symptoms, joint structures, and cartilage or bone biomarkers in patients with knee osteoarthritis

    DOI: 10.1007/s10067-019-04718-8

    PMID: 31377918

    Sample: Serum
  4. The Angle Orthodontist (2019) IF: 1.88
    Comparison of BALP, CTX-I, and IL-4 levels around miniscrew implants during orthodontic tooth movement between two different amounts of force

    DOI: 10.2319/071718-520.1

    PMID: 30730198

    Sample: Gingival Crevicular Fluid
  5. Journal of Advances in Medicine and Medical Research (2016)
    Evaluation of Osteopontin in Combination with Bone Turnover Markers for the Assessment of Osteoporosis in Postmenopausal Women

    DOI: 10.9734/BJMMR/2016/24738

    PMID: 99999999

    Sample: Serum
  6. Journal of Dental Sciences (2019) IF: 0.619
    Diagnostic accuracy of salivary biomarkers of bone turnover in identifying patients with periodontitis in a Saudi Arabian population

    DOI: 10.1016/j.jds.2019.03.002

    PMID: 31534637

    Sample: Saliva
  7. Faculty of Applied Health Sciences, Brock University (2019)
    Sclerostin Response to Exercise: Association with Bone Turnover, Oxidative Stress and Inflammation

    PMID: 99999999

    Sample: Serum
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