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Human CTXⅠ(Cross Linked C-telopeptide of Type Ⅰ Collagen) ELISA Kit

Cat.No.:E-EL-H0835
Reactivity: Human

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Product Details

Properties

Assay type	Sandwich-ELISA
Format	96T/48T
Assay time	3.5h
Detection range	0.16-10 ng/mL
Sensitivity	0.10 ng/mL
Sample type &Sample volume	serum, plasma and other biological fluids; 100μL
Specificity	This kit recognizes Human CTXⅠ in samples. No significant cross-reactivity or interference between Human CTXⅠ and analogues was observed.
Reproducibility	Both intra-CV and inter-CV are < 10%.
Application	This ELISA kit applies to the in vitro quantitative determination of Human CTXⅠ concentrations in serum, plasma and other biological fluids.

Test Principle

This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Human CTXⅠ. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human CTXⅠ and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human CTXⅠ, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Human CTXⅠ. You can calculate the concentration of Human CTXⅠ in the samples by comparing the OD of the samples to the standard curve.

Kit components & Storage

An unopened kit can be stored at 2-8℃ for 1 month. If the kit is not supposed to be used within 1 month, store the components separately according to the following conditions once the kit is received.

Item	Specifications	Storage
Micro ELISA Plate(Dismountable)	96T: 8 wells ×12 strips 48T: 8 wells ×6 strips	-20℃, 6 months
Reference Standard	96T: 2 vials 48T: 1 vial
Concentrated Biotinylated Detection Ab (100×)	96T: 1 vial, 120 μL 48T: 1 vial, 60 μL
Concentrated HRP Conjugate (100×)	96T: 1 vial, 120 μL 48T: 1 vial, 60 μL	-20℃(Protect from light), 6 months
Reference Standard & Sample Diluent	1 vial, 20 mL	2-8°C, 6 months
Biotinylated Detection Ab Diluent	1 vial, 14 mL
HRP Conjugate Diluent	1 vial, 14 mL
Concentrated Wash Buffer (25×)	1 vial, 30 mL
Substrate Reagent	1 vial, 10 mL	2-8℃(Protect from light)
Stop Solution	1 vial, 10 mL	2-8°C
Plate Sealer	5 pieces
Manual	1 copy
Certificate of Analysis	1 copy

Technical Data

Precision

Intra-assay Precision (Precision within an assay): 3 samples with low, mid range and high level Human CTXⅠ were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, mid range and high level Human CTXⅠ were tested on 3 different plates, 20 replicates in each plate.

	Intra-assay Precision			Inter-assay Precision
Sample	1	2	3	1	2	3
n	20	20	20	20	20	20
Mean (ng/mL)	0.52	1.08	4.39	0.49	0.99	4.46
Standard deviation	0.04	0.06	0.23	0.03	0.05	0.21
CV (%)	7.69	5.56	5.24	6.12	5.05	4.71

Recovery

The recovery of Human CTXⅠ spiked at three different levels in samples throughout the range of the assay was evaluated in various matrices.

Sample Type	Range (%)	Average Recovery (%)
Serum(n=8)	88-101	93
EDTA plasma (n=8)	94-109	100
Cell culture media (n=8)	88-100	93

Linearity

Samples were spiked with high concentrations of Human CTXⅠ and diluted with Reference Standard & Sample Diluent to produce samples with values within the range of the assay.

Target Information

Synonyms	CTX-I, CTX1
Research Area	Metabolism, Cardiovascular, Cancer

Assay Procedures

	1. Add 100μL standard or sample to the wells. Incubate for 90 min at 37°C
	2. Discard the liquid, immediately add 100μL Biotinylated Detection Ab working solution to each well. Incubate for 60 min at 37°C
	3. Aspirate and wash the plate for 3 times
	4. Add 100μL HRP conjugate working solution. Incubate for 30 min at 37°C. Aspirate and wash the plate for 5 times
	5. Add 90μL Substrate Reagent. Incubate for 15 min at 37°C
	6. Add 50μL Stop Solution
	7. Read the plate at 450nm immediately. Calculation of the results

Citations

JOURNAL OF NUTRITIONAL BIOCHEMISTRY (2022) IF: 6.117
Maternal omega-3 LC-PUFA supplementation programs an improved bone mass in the offspring with a more pronounced effect in females than males at adulthood

DOI: 10.1016/j.jnutbio.2022.109245

Sample: plasma
Biomolecules (2020) IF: 4.082
Salivary Osteocalcin as Potential Diagnostic Marker of Periodontal Bone Destruction among Smokers

DOI: 10.3390/biom10030380

Sample: saliva
Human Cell (2022) IF: 4.374
METTL14 upregulates TCF1 through m6A mRNA methylation to stimulate osteogenic activity in osteoporosis

DOI: 10.1007/s13577-022-00825-y

Sample: serum
OSTEOARTHRITIS AND CARTILAGE (2017) IF: 4.742
Associations between serum ghrelin and knee symptoms, joint structures and cartilage or bone biomarkers in patients with knee osteoarthritis

DOI: 10.1016/j.joca.2017.05.015

Sample: Serum
CALCIFIED TISSUE INTERNATIONAL (2019) IF: 3.265
Effects of High-Intensity Interval Running Versus Cycling on Sclerostin, and Markers of Bone Turnover and Oxidative Stress in Young Men

DOI: 10.1007/s00223-019-00524-1

PMID: 30671591
Sample: Serum
Biomed Research International (2020) IF: 2.276
Osteokines and Bone Markers at Rest and following Plyometric Exercise in Pre- and Postmenopausal Women

DOI: 10.1155/2020/7917309

PMID: 33145358
Sample: Serum
CLINICAL RHEUMATOLOGY (2019) IF: 2.293
Associations between serum IL-8 and knee symptoms, joint structures, and cartilage or bone biomarkers in patients with knee osteoarthritis

DOI: 10.1007/s10067-019-04718-8

PMID: 31377918
Sample: Serum
Biomed Research International (2018) IF: 2.583
Response of Sclerostin and Bone Turnover Markers to High Intensity Interval Exercise in Young Women: Does Impact Matter?

DOI: 10.1155/2018/4864952

Sample: serum
Archives of Osteoporosis (2021) IF: 2.617
Age-related trends and reference intervals of cross-linked C-telopeptide of type I collagen and procollagen type I N-propeptide from a reference population of Sri Lankan adult women

DOI: 10.1007/s11657-021-01022-4

PMID: 34727246
Sample: serum
Children-Basel (2021) IF: 2.863
Circulating Levels of Bone Markers after Short-Term Intense Training with Increased Dairy Consumption in Adolescent Female Athletes

DOI: 10.3390/children8110961

Sample: serum
ANGLE ORTHODONTIST (2019) IF: 1.88
Comparison of BALP, CTX-I, and IL-4 levels around miniscrew implants during orthodontic tooth movement between two different amounts of force

DOI: 10.2319/071718-520.1

PMID: 30730198
Sample: Gingival Crevicular Fluid
Journal of Dental Sciences (2019) IF: 0.798
Diagnostic accuracy of salivary biomarkers of bone turnover in identifying patients with periodontitis in a Saudi Arabian population

DOI: 10.1016/j.jds.2019.03.002

PMID: 31534637
Sample: Saliva

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