Current Position:Home>>Kits>>ELISA Kits>> Human IL-17A(Interleukin 17A) ELISA Kit

Human IL-17A(Interleukin 17A) ELISA Kit

Citations(9)Uniprot : Q16552

Cat.No.:E-EL-H0105
Reactivity: Human

Filter:

ELISA

To Purchase E-EL-H0105

Size:

96T
48T
24T
96T*5
96T*10

Price: $495

Qty:

Welcome to order from local distributors.

Protocols for ELISA

FAQ for ELISA

Have Questions?

Add to cart Bulk request

For research use only. Order now, ship in 3 days

Product Details

Properties

Assay type	Sandwich-ELISA
Format	96T/48T
Assay time	3.5h
Detection range	31.25-2000 pg/mL
Sensitivity	18.75 pg/mL
Sample type &Sample volume	serum, plasma and other biological fluids; 100μL
Specificity	This kit recognizes Human IL-17A in samples. No significant cross-reactivity or interference between Human IL-17A and analogues was observed.
Reproducibility	Both intra-CV and inter-CV are < 10%.
Application	This ELISA kit applies to the in vitro quantitative determination of Human IL-17A concentrations in serum, plasma and other biological fluids.

Test Principle

This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Human IL-17A. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human IL-17A and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human IL-17A, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Human IL-17A. You can calculate the concentration of Human IL-17A in the samples by comparing the OD of the samples to the standard curve.

Kit components & Storage

An unopened kit can be stored at 2-8℃ for 1 month. If the kit is not supposed to be used within 1 month, store the components separately according to the following conditions once the kit is received.

Item	Specifications	Storage
Micro ELISA Plate(Dismountable)	96T: 8 wells ×12 strips 48T: 8 wells ×6 strips	-20℃, 6 months
Reference Standard	96T: 2 vials 48T: 1 vial
Concentrated Biotinylated Detection Ab (100×)	96T: 1 vial, 120 μL 48T: 1 vial, 60 μL
Concentrated HRP Conjugate (100×)	96T: 1 vial, 120 μL 48T: 1 vial, 60 μL	-20℃(Protect from light), 6 months
Reference Standard & Sample Diluent	1 vial, 20 mL	2-8°C, 6 months
Biotinylated Detection Ab Diluent	1 vial, 14 mL
HRP Conjugate Diluent	1 vial, 14 mL
Concentrated Wash Buffer (25×)	1 vial, 30 mL
Substrate Reagent	1 vial, 10 mL	2-8℃(Protect from light)
Stop Solution	1 vial, 10 mL	2-8°C
Plate Sealer	5 pieces
Manual	1 copy
Certificate of Analysis	1 copy

Technical Data

Precision

Intra-assay Precision (Precision within an assay): 3 samples with low, mid range and high level Human IL-17A were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, mid range and high level Human IL-17A were tested on 3 different plates, 20 replicates in each plate.

	Intra-assay Precision			Inter-assay Precision
Sample	1	2	3	1	2	3
n	20	20	20	20	20	20
Mean (pg/mL)	95.20	215.10	996.10	94.90	200.40	990.40
Standard deviation	6.30	10.10	37.90	6.00	9.20	50.50
CV (%)	6.62	4.70	3.80	6.32	4.59	5.10

Recovery

The recovery of Human IL-17A spiked at three different levels in samples throughout the range of the assay was evaluated in various matrices.

Sample Type	Range (%)	Average Recovery (%)
Serum(n=8)	87-99	93
EDTA plasma (n=8)	85-97	90
Cell culture media (n=8)	86-101	93

Linearity

Samples were spiked with high concentrations of Human IL-17A and diluted with Reference Standard & Sample Diluent to produce samples with values within the range of the assay.

Target Information

Database Links	SwissProt: Q16552
Synonyms	CTLA8, CTLA-8, CTLA8cytotoxic T-lymphocyte-associated serine esterase 8, Cytotoxic T-lymphocyte-associated antigen 8, IL17, IL-17, IL17A, IL-17A, IL-17Acytotoxic T-lymphocyte-associated protein 8, IL-17CTLA-8
Research Area	Cancer, Immunology

Assay Procedures

	1. Add 100μL standard or sample to the wells. Incubate for 90 min at 37°C
	2. Discard the liquid, immediately add 100μL Biotinylated Detection Ab working solution to each well. Incubate for 60 min at 37°C
	3. Aspirate and wash the plate for 3 times
	4. Add 100μL HRP conjugate working solution. Incubate for 30 min at 37°C. Aspirate and wash the plate for 5 times
	5. Add 90μL Substrate Reagent. Incubate for 15 min at 37°C
	6. Add 50μL Stop Solution
	7. Read the plate at 450nm immediately. Calculation of the results

Citations

From now on, if you have published a paper by using any of our products since 1/1/2019, fill out the “Elabscience Publication Reward Application Form”carefully and send it to orders@elabscience.com, we will get back to you with the reward after we confirm it ASAP!

View more details about our Publication Reward >>

Theranostics (2021) IF: 11.556

A SCID mouse-human lung xenograft model of SARS-CoV-2 infection

DOI: 10.7150/thno.58321
PMID: 33995679	Sample: Tissue homogenate

Scandinavian Journal of Immunology (2020) IF: 2.717

Early HIV infection is associated with reduced proportions of gamma delta T subsets as well as high creatinine and urea levels

DOI: 10.1111/sji.12868
PMID: 32052490	Sample: Serum

Bioscience reports (2018) IF: 2.899

Correlations of IL-17 and NF-κB Gene Polymorphisms With Susceptibility and Prognosis in Acute Respiratory Distress Syndrome in a Chinese Population

DOI: 10.1042/BSR20181987
PMID: 30655311	Sample: Serum

Journal of Cosmetic Dermatology (2019) IF: 1.621

Serum interleukin-17 as a novel biomarker in patients with acne vulgaris

DOI: 10.1111/jocd.12934
PMID: 30964235	Sample: Serum

AIP Conference Proceedings (2020)

Correlation study of (IL-17, IL-17RA) and some biochemical parameters with patients of Iraq colorectal cancer after and before taking a dose of chemotherapy

DOI: 10.1063/5.0000066
	Sample: Serum

Asian Journal of Microbiology, Biotechnology and Environmental Sciences (2018)

Evaluation of serum level of interleukin-17 among pulmonary tuberculosis patients in Babylon province


	Sample: Serum

Indian Journal of Forensic Medicine & Toxicology (2019)

Influence of TLR4 Polymorphisms on Susceptibility to UTI Caused by Gram Negative Bacteria in Pregnant Women

DOI: 10.5958/0973-9130.2019.00454.7
	Sample: Serum,Urine

Journal of the Egyptian Women's Dermatologic Society (2019)

Interleukin-17 levels in patients with psoriasis with or without metabolic syndrome

DOI: 10.4103/JEWD.JEWD_29_19
	Sample: Serum

EurAsian Journal of BioSciences (2020)

Interleukin-17 serum levels as a vital indicator of psoriatic Iraqi patients in Thi-Qar Province


	Sample: Serum

Indian Journal of Public Health (2020)

Association of TLR4 Polymorphisms with Susceptibility to Urinary Tract Infection Caused by Gram Positive Bacteria in Pregnant Women


	Sample: Serum

Show AllShorten

Reviews/Q&A

Show all
Reviews
Q&A

a**nSubmitted [ Aug 30 2022 ]

Asked: What is the optimum dilution fold for the serum sample?

adminSubmitted [ Aug 30 2022 ]

Answered: For E-EL-H0105, the optimum dilution fold for the serum sample is 1:1.

... Show All Show Less

*Product Name:
*Catalog Number:
Size:
*Name:
*Email:
*Phone:
*Message:
*Country:
*When will you use it?	Within 1 month In 2 to 3 month In 4 to 6 month 6 months later
*Captcha:	Click to load Captcha