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Human ROMO1(Reactive oxygen species modulator 1) ELISA Kit

Citations(3)Uniprot : P60602

Cat.No.:E-EL-H5430
Reactivity: Human

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Product Details

Properties

Assay type	Sandwich-ELISA
Format	96T/48T
Assay time	3.5h
Detection range	0.16-10 ng/mL
Sensitivity	0.10 ng/mL
Sample type &Sample volume	serum, plasma and other biological fluids; 100μL
Specificity	This kit recognizes Human ROMO1 in samples. No significant cross-reactivity or interference between Human ROMO1 and analogues was observed.
Reproducibility	Both intra-CV and inter-CV are < 10%.
Application	This ELISA kit applies to the in vitro quantitative determination of Human ROMO1 concentrations in serum, plasma and other biological fluids.

Test Principle

This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Human ROMO1. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human ROMO1 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human ROMO1, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Human ROMO1. You can calculate the concentration of Human ROMO1 in the samples by comparing the OD of the samples to the standard curve.

Kit components & Storage

An unopened kit can be stored at 2-8℃ for 1 month. If the kit is not supposed to be used within 1 month, store the components separately according to the following conditions once the kit is received.

Item	Specifications	Storage
Micro ELISA Plate(Dismountable)	96T: 8 wells ×12 strips 48T: 8 wells ×6 strips	-20℃, 6 months
Reference Standard	96T: 2 vials 48T: 1 vial
Concentrated Biotinylated Detection Ab (100×)	96T: 1 vial, 120 μL 48T: 1 vial, 60 μL
Concentrated HRP Conjugate (100×)	96T: 1 vial, 120 μL 48T: 1 vial, 60 μL	-20℃(Protect from light), 6 months
Reference Standard & Sample Diluent	1 vial, 20 mL	2-8°C, 6 months
Biotinylated Detection Ab Diluent	1 vial, 14 mL
HRP Conjugate Diluent	1 vial, 14 mL
Concentrated Wash Buffer (25×)	1 vial, 30 mL
Substrate Reagent	1 vial, 10 mL	2-8℃(Protect from light)
Stop Solution	1 vial, 10 mL	2-8°C
Plate Sealer	5 pieces
Manual	1 copy
Certificate of Analysis	1 copy

Technical Data

Precision

Intra-assay Precision (Precision within an assay): 3 samples with low, mid range and high level Human ROMO1 were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, mid range and high level Human ROMO1 were tested on 3 different plates, 20 replicates in each plate.

	Intra-assay Precision			Inter-assay Precision
Sample	1	2	3	1	2	3
n	20	20	20	20	20	20
Mean (ng/mL)	0.47	1.29	4.00	0.49	1.27	3.66
Standard deviation	0.03	0.05	0.19	0.03	0.07	0.11
CV (%)	6.38	3.88	4.75	6.12	5.51	3.01

Recovery

The recovery of Human ROMO1 spiked at three different levels in samples throughout the range of the assay was evaluated in various matrices.

Sample Type	Range (%)	Average Recovery (%)
Serum(n=8)	86-97	92
EDTA plasma (n=8)	92-108	99
Cell culture media (n=8)	88-102	95

Linearity

Samples were spiked with high concentrations of Human ROMO1 and diluted with Reference Standard & Sample Diluent to produce samples with values within the range of the assay.

Target Information

Database Links	SwissProt: P60602
Research Area	Cancer, Cell Biology, Epigenetics and Nuclear Signaling, Metabolism, Microbiology, Signal transduction

Assay Procedures

	1. Add 100μL standard or sample to the wells. Incubate for 90 min at 37°C
	2. Discard the liquid, immediately add 100μL Biotinylated Detection Ab working solution to each well. Incubate for 60 min at 37°C
	3. Aspirate and wash the plate for 3 times
	4. Add 100μL HRP conjugate working solution. Incubate for 30 min at 37°C. Aspirate and wash the plate for 5 times
	5. Add 90μL Substrate Reagent. Incubate for 15 min at 37°C
	6. Add 50μL Stop Solution
	7. Read the plate at 450nm immediately. Calculation of the results

Citations

Advanced Fiber Materials (2023) IF: 12.958
Double-Layer Nanofibrous Sponge Tube via Electrospun Fiber and Yarn for Promoting Urethral Regeneration

DOI: 10.1007/s42765-022-00252-6

Sample: Human foreskin fibroblasts (HFFs),HSF cell
Journal of Functional Foods (2017) IF: 3.144
Antioxidant and DNA protective effects of NTX, a proprietary glycyrrhizin/d-mannitol product, in association with alcohol consumption: A randomized, placebo-controlled, double-blind, crossover study

DOI: 10.1016/j.jff.2017.04.011

Sample: testes
ARCHIVES OF GYNECOLOGY AND OBSTETRICS (2022) IF: 2.344
Diagnostic and prognostic role of TFF3, Romo-1, NF-кB and SFRP4 as biomarkers for endometrial and ovarian cancers: a prospective observational translational study

DOI: 10.1007/s00404-022-06563-8

PMID: 35461390
Sample: serum

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Reviews/Q&A

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Q&A

i********tSubmitted [ Nov 12 2018 ]

Asked: Hello, Are there any publications done using this ELISA kit that can be used as reference and since when has this kit been produced? Thank you in advance

adminSubmitted [ Nov 12 2018 ]

Answered: Dear Ipek Thank you for your mail. For this elisa kit, there is no publication, but we have in-house data for reference. the reference values for HeLa cell lysates were 5.33~21.62ng/mL, The HeLa cells were cultured in RPMI-1640＋10% FBS＋1% P/S for 3 days. Any other inquiry,please feel free to contact us. Best regards

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