Ramos [RA 1] Cell Line

Name: Ramos [RA 1] Cell Line
Brand: Elabscience
SKU: CL-0483
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Cell Name	Ramos [RA 1] Cell Line
Organism	Homo sapiens, Human
Age	3 years
Tissue	Peripheral blood
Morphology	Lymphoblast
Growth Properties	Suspension
Descriptions	The cells are negative for Epstein-Barr virus. Both the parental line and the derivative cell line Ramos. 2G6. 4C10 have about 1500 IL-4 binding sites per cell. The cells are reported to secrete IgM(lambda light chain).
Biosafety Level	1

Complete Growth Medium	RPMI-1640(PM150110)+10% FBS+1% P/S(PB180120) (Ramos [RA 1] Cell Line complete medium (CM-0483) is recommended.)
Subculturing	Cultures can be maintained by the addition of fresh medium or replacement of medium. Alternatively, cultures can be established by centrifugation with subsequent resuspension at the subcultivation ratio of 1:3-1:5.
Subcultivation Ratio	1:2 to 1:4
Medium Renewal	Every 2 to 3 days
Cryopreservation	Freeze medium: 60% Basal medium+30% FBS+10% DMSOStorage temperature: Liquid nitrogen vapor phase
Culture Conditions	Atmosphere: Air, 95%; CO2, 5%Temperature: 37℃

Tumorigenic	Yes
Effects	Yes, forms tumors in nude mice.
Receptor Expression	Interleukin 4(IL-4); low affinity IgE(CD23)
Antigen Expression	CD23＋
Gene Expression	Immunoglobulin(Surface And Secreted)
Applications	Transfection.

PM150110P	RPMI-1640, powder
PM150110	RPMI-1640
PB180120	Penicillin-Streptomycin Solution, 100×
PB180327	Phosphate Buffer (PBS)
PB180419	200 mM L-Alanyl-L-Glutamine Solution, 100×

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