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Rat ALB(Albumin) ELISA Kit

Citations(10)Uniprot : P02770

Cat.No.:E-EL-R0362
Reactivity: Rat

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96T
48T
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96T*5
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Product Details

Properties

Assay type	Sandwich-ELISA
Format	96T/48T
Assay time	3.5h
Detection range	3.13-200 μg/mL
Sensitivity	1.88 μg/mL
Sample type &Sample volume	serum, plasma and other biological fluids; 100μL
Specificity	This kit recognizes Rat ALB in samples. No significant cross-reactivity or interference between Rat ALB and analogues was observed.
Reproducibility	Both intra-CV and inter-CV are < 10%.
Application	This ELISA kit applies to the in vitro quantitative determination of Rat ALB concentrations in serum, plasma and other biological fluids.

Test Principle

This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Rat ALB. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Rat ALB and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Rat ALB, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Rat ALB. You can calculate the concentration of Rat ALB in the samples by comparing the OD of the samples to the standard curve.

Kit components & Storage

An unopened kit can be stored at 2-8℃ for 1 month. If the kit is not supposed to be used within 1 month, store the components separately according to the following conditions once the kit is received.

Item	Specifications	Storage
Micro ELISA Plate(Dismountable)	96T: 8 wells ×12 strips 48T: 8 wells ×6 strips	-20℃, 12 months
Reference Standard	96T: 2 vials 48T: 1 vial
Concentrated Biotinylated Detection Ab (100×)	96T: 1 vial, 120 μL 48T: 1 vial, 60 μL
Concentrated HRP Conjugate (100×)	96T: 1 vial, 120 μL 48T: 1 vial, 60 μL	-20℃(Protect from light), 12 months
Reference Standard & Sample Diluent	1 vial, 20 mL	2-8°C, 12 months
Biotinylated Detection Ab Diluent	1 vial, 14 mL
HRP Conjugate Diluent	1 vial, 14 mL
Concentrated Wash Buffer (25×)	1 vial, 30 mL
Substrate Reagent	1 vial, 10 mL	2-8℃(Protect from light)
Stop Solution	1 vial, 10 mL	2-8°C
Plate Sealer	5 pieces
Manual	1 copy
Certificate of Analysis	1 copy

Technical Data

Precision

Intra-assay Precision (Precision within an assay): 3 samples with low, mid range and high level Rat ALB were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, mid range and high level Rat ALB were tested on 3 different plates, 20 replicates in each plate.

	Intra-assay Precision			Inter-assay Precision
Sample	1	2	3	1	2	3
n	20	20	20	20	20	20
Mean (μg/mL)	9.90	25.50	76.00	9.80	23.30	78.50
Standard deviation	0.50	1.20	3.60	0.50	1.20	2.70
CV (%)	5.05	4.71	4.74	5.10	5.15	3.44

Recovery

The recovery of Rat ALB spiked at three different levels in samples throughout the range of the assay was evaluated in various matrices.

Sample Type	Range (%)	Average Recovery (%)
Serum(n=8)	86-96	91
EDTA plasma (n=8)	86-100	91
Cell culture media (n=8)	95-107	101

Linearity

Samples were spiked with high concentrations of Rat ALB and diluted with Reference Standard & Sample Diluent to produce samples with values within the range of the assay.

Target Information

Database Links	SwissProt: P02770
Research Area	Cancer, Cardiovascular, Metabolism, Developmental biology, Signal transduction, Stem cells

Assay Procedures

	1. Add 100μL standard or sample to the wells. Incubate for 90 min at 37°C
	2. Discard the liquid, immediately add 100μL Biotinylated Detection Ab working solution to each well. Incubate for 60 min at 37°C
	3. Aspirate and wash the plate for 3 times
	4. Add 100μL HRP conjugate working solution. Incubate for 30 min at 37°C. Aspirate and wash the plate for 5 times
	5. Add 90μL Substrate Reagent. Incubate for 15 min at 37°C
	6. Add 50μL Stop Solution
	7. Read the plate at 450nm immediately. Calculation of the results

Citations

DRUG DEVELOPMENT RESEARCH (2019) IF: 1.742

Montelukast modifies simvastatin‐induced myopathy and hepatotoxicity

DOI: 10.1002/ddr.21581
	Sample: Liver

JOURNAL OF FOOD BIOCHEMISTRY (2022) IF: 2.72

Watermelon (Citrullus lanatus) leaf extract attenuates biochemical and histological parameters in high-fat diet/streptozotocin-induced diabetic rats

DOI: 10.1111/jfbc.14058
PMID: 34981526	Sample: Serum

Animals (2022) IF: 2.752

Effect of TMR Briquettes on Milk Production, Nutrient Digestibility, and Manure Excretions of Dairy Cows in the Dry Zone of Sri Lanka

DOI: 10.3390/ani12070932
PMID: 35405920	Sample: Serum

GENE (2020) IF: 2.984

Chitosan-stabilized selenium nanoparticles alleviate cardio-hepatic damage in type 2 diabetes mellitus model via regulation of caspase, Bax/Bcl-2, and Fas/FasL-pathway

DOI: 10.1016/j.gene.2020.145288
PMID: 33181259	Sample: Serum

Frontiers in Physiology (2019) IF: 3.201

Protective Effect of Ursolic Acid on the Intestinal Mucosal Barrier in a Rat Model of Liver Fibrosis

DOI: 10.3389/fphys.2019.00956
	Sample: Liver,Ileum

BIOCHIMICA ET BIOPHYSICA ACTA-MOLECULAR CELL RESEARCH (2020) IF: 4.105

Cathepsin C is a novel mediator of podocyte and renal injury induced by hyperglycemia

DOI: 10.1016/j.bbamcr.2020.118723
	Sample: Urine

JOURNAL OF MOLECULAR MEDICINE-JMM (2022) IF: 4.599

PTEN-induced kinase 1 deficiency alters albumin permeability and insulin signaling in podocytes

DOI: 10.1007/s00109-022-02204-4
PMID: 35534645	Sample: Urinary

BIOCHIMICA ET BIOPHYSICA ACTA-MOLECULAR CELL RESEARCH (2022) IF: 4.739

Insulin controls cytoskeleton reorganization and filtration barrier permeability via the PKGIα-Rac1-RhoA crosstalk in cultured rat podocytes

DOI: 10.1016/j.bbamcr.2022.119301
PMID: 35642843	Sample: Urine

Food & Function (2022) IF: 5.396

Asiatic acid from Cyclocarya paliurus regulates the autophagy–lysosome system via directly inhibiting TGF-β type I receptor and ameliorates diabetic nephropathy fibrosis

DOI: 10.1039/D1FO02445K
PMID: 35531774	Sample: Urinary

Stem Cell Research & Therapy (2022) IF: 8.079

Therapeutic potential of conditioned medium obtained from deferoxamine preconditioned umbilical cord mesenchymal stem cells on diabetic nephropathy model

DOI: 10.1186/s13287-022-03121-6
PMID: 36056427	Sample: Urine

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