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Rat ALB(Albumin) ELISA Kit

  • Cat.No.:E-EL-R0362

  • Reactivity: Rat

To Purchase E-EL-R0362

  • 96T
  • 48T
  • 24T
  • 96T*5
  • 96T*10
Price: $495

Product Details


Assay type Sandwich-ELISA
Format 96T/48T
Assay time 3.5h
Detection range 3.13-200 μg/mL
Sensitivity 1.88 μg/mL
Sample type &Sample volume serum, plasma and other biological fluids; 100μL
Specificity This kit recognizes Rat ALB in samples. No significant cross-reactivity or interference between Rat ALB and analogues was observed.
Reproducibility Both intra-CV and inter-CV are < 10%.
Application This ELISA kit applies to the in vitro quantitative determination of Rat ALB concentrations in serum, plasma and other biological fluids.

Test Principle

This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Rat ALB. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Rat ALB and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Rat ALB, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Rat ALB. You can calculate the concentration of Rat ALB in the samples by comparing the OD of the samples to the standard curve.

Kit components & Storage

An unopened kit can be stored at 2-8℃ for 1 month. If the kit is not supposed to be used within 1 month, store the components separately according to the following conditions once the kit is received.
Micro ELISA Plate(Dismountable) 96T: 8 wells ×12 strips
48T: 8 wells ×6 strips
-20℃, 6 months
Reference Standard 96T: 2 vials
48T: 1 vial
Concentrated Biotinylated Detection Ab (100×) 96T: 1 vial, 120 μL
48T: 1 vial, 60 μL
Concentrated HRP Conjugate (100×) 96T: 1 vial, 120 μL
48T: 1 vial, 60 μL
-20℃(Protect from light), 6 months
Reference Standard & Sample Diluent 1 vial, 20 mL 2-8°C, 6 months
Biotinylated Detection Ab Diluent 1 vial, 14 mL
HRP Conjugate Diluent 1 vial, 14 mL
Concentrated Wash Buffer (25×) 1 vial, 30 mL
Substrate Reagent 1 vial, 10 mL 2-8℃(Protect from light)
Stop Solution 1 vial, 10 mL 2-8°C
Plate Sealer 5 pieces
Manual 1 copy
Certificate of Analysis 1 copy

Technical Data


Intra-assay Precision (Precision within an assay): 3 samples with low, mid range and high level Rat ALB were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, mid range and high level Rat ALB were tested on 3 different plates, 20 replicates in each plate.

  Intra-assay Precision Inter-assay Precision
Sample 1 2 3 1 2 3
n 20 20 20 20 20 20
Mean (μg/mL) 9.90 25.50 76.00 9.80 23.30 78.50
Standard deviation 0.50 1.20 3.60 0.50 1.20 2.70
CV (%) 5.05 4.71 4.74 5.10 5.15 3.44


The recovery of Rat ALB spiked at three different levels in samples throughout the range of the assay was evaluated in various matrices.

Sample Type Range (%) Average Recovery (%)
Serum(n=8) 86-96 91
EDTA plasma (n=8) 86-100 91
Cell culture media (n=8) 95-107 101


Samples were spiked with high concentrations of Rat ALB and diluted with Reference Standard & Sample Diluent to produce samples with values within the range of the assay.

Target Information

Assay Procedures

elisa assay procedure 1

1. Add 100μL standard or sample to the wells. Incubate for 90 min at 37°C

elisa assay procedure 2

2. Discard the liquid, immediately add 100μL Biotinylated Detection Ab working solution to each well. Incubate for 60 min at 37°C

elisa assay procedure 3

3. Aspirate and wash the plate for 3 times

elisa assay procedure 4

4. Add 100μL HRP conjugate working solution. Incubate for 30 min at 37°C. Aspirate and wash the plate for 5 times

elisa assay procedure 5

5. Add 90μL Substrate Reagent. Incubate for 15 min at 37°C

elisa assay procedure 6

6. Add 50μL Stop Solution

elisa assay procedure 7

7. Read the plate at 450nm immediately. Calculation of the results


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View more details about our Publication Reward >>

  1. Stem Cell Research & Therapy (2022) IF: 8.0790
    Therapeutic potential of conditioned medium obtained from deferoxamine preconditioned umbilical cord mesenchymal stem cells on diabetic nephropathy model

    DOI: 10.1186/s13287-022-03121-6

    PMID: 36056427

    Sample: Urine
  2. Food & Function (2022) IF: 5.396
    Asiatic acid from Cyclocarya paliurus regulates the autophagy–lysosome system via directly inhibiting TGF-β type I receptor and ameliorates diabetic nephropathy fibrosis

    DOI: 10.1039/D1FO02445K

    PMID: 35531774

    Sample: Urinary
    Cathepsin C is a novel mediator of podocyte and renal injury induced by hyperglycemia

    DOI: 10.1016/j.bbamcr.2020.118723

    Sample: Urine
    PTEN-induced kinase 1 deficiency alters albumin permeability and insulin signaling in podocytes

    DOI: 10.1007/s00109-022-02204-4

    PMID: 35534645

    Sample: Urinary
    Insulin controls cytoskeleton reorganization and filtration barrier permeability via the PKGIα-Rac1-RhoA crosstalk in cultured rat podocytes

    DOI: 10.1016/j.bbamcr.2022.119301

    PMID: 35642843

    Sample: urine
  6. Frontiers in Physiology (2019) IF: 3.201
    Protective Effect of Ursolic Acid on the Intestinal Mucosal Barrier in a Rat Model of Liver Fibrosis

    DOI: 10.3389/fphys.2019.00956

    Sample: liver,ileum
    Watermelon (Citrullus lanatus) leaf extract attenuates biochemical and histological parameters in high-fat diet/streptozotocin-induced diabetic rats

    DOI: 10.1111/jfbc.14058

    PMID: 34981526

    Sample: serum
  8. Animals (2022) IF: 2.752
    Effect of TMR Briquettes on Milk Production, Nutrient Digestibility, and Manure Excretions of Dairy Cows in the Dry Zone of Sri Lanka

    DOI: 10.3390/ani12070932

    PMID: 35405920

    Sample: serum
  9. GENE (2020) IF: 2.984
    Chitosan-stabilized selenium nanoparticles alleviate cardio-hepatic damage in type 2 diabetes mellitus model via regulation of caspase, Bax/Bcl-2, and Fas/FasL-pathway

    DOI: 10.1016/j.gene.2020.145288

    PMID: 33181259

    Sample: Serum
    Montelukast modifies simvastatin‐induced myopathy and hepatotoxicity

    DOI: 10.1002/ddr.21581

    Sample: liver
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