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Cat.No.:PKSH033388
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Synonyms | T-Cell Surface Glycoprotein CD3 Epsilon Chain;T-Cell Surface Antigen T3/Leu-4 Epsilon Chain;CD3e;CD3E;T3E;CD3 epsilon;IMD18 |
Species | Human |
Expression_host | HEK293 Cells |
Sequence | Asp23-Asp126 |
Accession | P07766 |
Application | Functional ELISA |
Mol_Mass | 12.8 kDa |
AP_Mol_Mass | 15-21 kDa |
Tag | C-His |
Bio_Activity | Immobilized Human CD3E-His at 10 ug/ml (100 ul/wel)can bind Human Anti-CD3 mAb. The ED50 of Recombinant Human Anti-CD3 mAb is 3-15 ng/ml. |
Purity | > 95 % as determined by reducing SDS-PAGE. |
Endotoxin level | < 1.0 EU per μg of the protein as determined by the LAL method. |
Storage | Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80℃. Reconstituted protein solution can be stored at 4-8℃ for 2-7 days. Aliquots of reconstituted samples are stable at < -20℃ for 3 months. |
Shipping | This product is provided as lyophilized powder which is shipped with ice packs. |
Formulation | Lyophilized from a 0.2 μm filtered solution of PBS, pH 7.4. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization. Please refer to the specific buffer information in the printed manual. |
Reconstitution | Please refer to the printed manual for detailed information. |