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Recombinant Human Protamine-2/PRM2 Protein (His Tag)

Uniprot : P31350

Cat.No.:PKSH032952
Expression host: HEK293 Cells

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Description

Synonyms	Ribonucleoside-Diphosphate Reductase Subunit M2;Ribonucleotide Reductase Small Chain;Ribonucleotide Reductase Small Subunit;RRM2;RR2
Species	Human
Expression_host	HEK293 Cells
Sequence	Met 1-Phe389
Accession	P31350
Mol_Mass	45.9 kDa
AP_Mol_Mass	47 kDa
Tag	C-His

Properties

Purity	> 95 % as determined by reducing SDS-PAGE.
Endotoxin level	< 1.0 EU per μg of the protein as determined by the LAL method.
Storage	Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80℃. Reconstituted protein solution can be stored at 4-8℃ for 2-7 days. Aliquots of reconstituted samples are stable at < -20℃ for 3 months.
Shipping	This product is provided as lyophilized powder which is shipped with ice packs.
Formulation	Lyophilized from a 0.2 μm filtered solution of 20mM Tris-HCl, 150mM NaCl, 5% Trehalose, pH 8.0. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization. Please refer to the specific buffer informatio
Reconstitution	Please refer to the printed manual for detailed information.

Background

Ribonucleoside-Diphosphate Reductase Subunit M2 (RRM2) belongs to the ribonucleoside diphosphate reductase small chain family. The reductase of RRM2 catalyzes the formation of deoxyribonucleotides from ribonucleotides. Synthesis of the encoded protein (M2) is regulated in a cell-cycle dependent fashion. RRM2 supplies the precursors essential for DNA synthesis. RRM2 catalyzes the biosynthesis of deoxyribonucleotides from the corresponding ribonucleotides. Phosphorylation on Ser-20 relieves the inhibitory effect on Wnt signaling.

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