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Recombinant Human PSA/KLK3 Protein (aa 25-261, His Tag)

Uniprot : P07288

Cat.No.:PKSH032949
Expression host: HEK293 Cells

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Description

Synonyms	APS;Hk3;KLK2A1;PSA;Prostate-Specific Antigen;PSA;Gamma-Seminoprotein;Seminin;Kallikrein-3;P-30 Antigen;Semenogelase;
Species	Human
Expression_host	HEK293 Cells
Sequence	Ile25-Pro261
Accession	P07288
Mol_Mass	27.1 kDa
AP_Mol_Mass	35 kDa
Tag	C-His

Properties

Purity	> 95 % as determined by reducing SDS-PAGE.
Endotoxin level	< 1.0 EU per μg of the protein as determined by the LAL method.
Storage	Store at < -20°C, stable for 6 months. Please minimize freeze-thaw cycles.
Shipping	This product is provided as liquid. It is shipped at frozen temperature with blue ice/gel packs. Upon receipt, store it immediately at < - 20°C.
Formulation	Supplied as a 0.2 μm filtered solution of 50mM Tris-HCl, 10mM CaCl₂, 150mM NaCl, 0.05% Brij 35, pH 7.5.
Reconstitution	Not Applicable

Background

KLK3; also known as APS; is short for Prostate-specific antigen. It is a 261 aa. protein which belongs to the peptidase S1 family and Kallikrein subfamily. This protein has 5 isforms produced by alternative splicing. It is a secreted protein and can forms a heterodimer with SERPINA5 which can inhibit its activity. KLK3 is also strongly inhibited by Zn2+; 100 times more abundant in semen than in serum. This inhibition is relieved by exposure to semenogelins; which are avid zinc binders. KLK3 can hydrolyzes semenogelin-1 thus leading to the liquefaction of the seminal coagulum.

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