VB12 microtiter plate test is a
microbiological method for the quantitative determination of VB12 microtiter
plate test is a microbiological method for the quantitative determination of
VB12 microtiter plate test is a microbiological method for the quantitative
determination of VB12 in food products. The microbiological test system is in
accordance with AOAC international norms.
VB12 is extracted from the sample and the
extract is diluted. The VB12 assay-medium and the diluted extract are pipetted
into the wells of a microtiter plate which is coated with Lactobacillus
delbriickii. The growth of Lactobacillus delbriickii is dependent on the supply
of VB12. Following the addition of VB12 as a standard or as a compound of the
sample, the bacteria grow until the vitamin is consumed. The incubation is done
in the dark at 37 °C (98.6 °F) for 44 - 48 h.
The intensity of metabolism or growth of Lactobacillus
delbriickii in relation to the extracted VB12 is measured as turbidity and
compared to a standard curve. The measurement is done using a microtiter plate
reader at 610 - 630 nm (alternatively at 540 - 550 nm).
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