VB9 microtiter plate test is a
microbiological method for the quantitative determination of VB9 microtiter
plate test is a microbiological method for the quantitative determination of VB9
microtiter plate test is a microbiological method for the quantitative
determination of VB9 in food products. The microbiological test system is in
accordance with AOAC international norms.
VB9 is extracted from the sample and the
extract is diluted. The VB9 assay-medium and the diluted extract are pipetted
into the wells of a microtiter plate which is coated with Lactobacillus
rhamnosus. The growth of Lactobacillus rhamnosus is dependent on the supply of VB9.
Following the addition of VB9 as a standard or as a compound of the sample, the
bacteria grow until the vitamin is consumed. The incubation is done in the dark
at 37 °C (98.6 °F) for 44 - 48 h.
The intensity of metabolism or growth of Lactobacillus
rhamnosus in relation to the extracted VB9 is measured as turbidity and
compared to a standard curve. The measurement is done using a microtiter plate
reader at 610 - 630 nm (alternatively at 540 - 550 nm).
Message submitted successfully !