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Alanine Aminotransferase (ALT/GPT) Activity Assay Kit

  • Cat.No.:E-BC-K235-S

  • Detection instrument: Spectrophotometer(505 nm)

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Price: $180

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Detection principle

ALT catalyze the amino conversion reaction between alanine and α-ketoglutaric acid to produce pyruvic acid and glutamic acid at pH 7.4 and 37℃. Then phenylhydrazine was added to form phenylhydrazone with pyruvic acid. Phenylhydrazone is reddish brown under alkaline conditions. ALT activity can be calculated by measuring the OD values at 510 nm.

Performance characteristics

Synonyms ALT,GPT,Glutamic-pyruvic transaminase
Sample type Serum,plasma,cells,animal tissue
Sensitivity 1.26 IU/L
Detection range 1.26-72.3 IU/L
Detection method Colorimetric method
Assay type Enzyme Activity
Assay time 100 min
Precision Average inter-assay CV: 8.200%Average intra-assay CV: 4.300%
Other instruments required Micropipettor, Vortex mixer, Incubator
Other reagents required Normal saline (0.9% NaCl), PBS (0.01 M, pH 7.4)
Storage 2-8℃
Valid period 12 months

Dilution of sample

It is recommended to take 2~3 samples with expected large difference to do pre-experiment before formal experiment and dilute the sample according to the result of the pre-experiment and the detection range (1.26-72.3 IU/L).

The recommended dilution factor for different samples is as follows (for reference only):

Sample type

Dilution factor

10% Rat liver tissue homogenization


10% Rat kidney tissue homogenization


Human serum


Human plasma


HepG2 cells homogenization


Note: The diluent is normal saline (0.9% NaCl) or PBS (0.01 M, pH 7.4).


  1. Cells (2021) IF: 6.6
    Indole-3-Carboxaldehyde Restores Gut Mucosal Integrity and Protects from Liver Fibrosis in Murine Sclerosing Cholangitis

    DOI: 10.3390/cells10071622

    PMID: 34209524

    Sample: serum
  2. PLoS Pathogens (2021) IF: 6.823
    IRF3-mediated pathogenicity in a murine model of human hepatitis A

    DOI: 10.1371/journal.ppat.1009960

    PMID: 34591933

    Sample: serum
    Sub-chronic oral toxicity evaluation of herbo-metallic formulation Arshakuthar rasa in rats

    DOI: 10.1016/j.jep.2022.115306

    PMID: 35443217

    Sample: serum
  4. BMC Complementary and Alternative Medicine (2022) IF: 3.659
    Artemisia argyi extract alleviates inflammation in a DSS-induced colitis mouse model and enhances immunomodulatory effects in lymphoid tissues

    DOI: 10.1186/s12906-022-03536-x

    PMID: 35277165

    Sample: serum
  5. PeerJ (2022) IF: 2.984
    3,4-dihydroxyphenylethyl alcohol glycoside reduces acetaminophen-induced acute liver failure in mice by inhibiting hepatocyte ferroptosis and pyroptosis

    DOI: 10.7717/peerj.13082

    PMID: 35310165

    Sample: plasma
  6. Journal of Nanomaterials (2021) IF: 2.986
    Effect of Hafnium Coating on Osseointegration of Titanium Implants: A Split Mouth Animal Study

    DOI: 10.1155/2021/7512957

    Sample: mandibular bone
    Toxic effects of subchronic oral acetamiprid exposure in rats:

    DOI: 10.1177/0748233719893203

    Sample: plasma
  8. Toxicology Reports (2017)
    Diclofenac – induced hepatotoxicity: Low dose of omega-3 fatty acids have more protective effects

    DOI: 10.1016/j.toxrep.2017.12.002

    Sample: plasma
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