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One-step TUNEL In Situ Apoptosis Kit (Red, Elab Fluor^® 647)

Cat.No.:E-CK-A324

Applications:Apoptosis/DNA Fragmentation

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Detection principle

When cells undergo apoptosis, specific DNA endonucleases will be activated, cutting the genomic DNA between the nucleosomes. The DNA of apoptotic cells is cleaved into multimers of 180~200bp fragments, corresponding to the oligonucleosomal size. Therefore, the DNA of apoptotic cells typically migrates as a ladder of 180~200bp on an agarose gel. The exposed 3'-OH of the broken DNA can be catalyzed by Terminal Deoxynucleotidyl Transferase (TdT) with fluorescein labeled dUTP, which can be detected with fluorescence microscope.

General Information

Application	DNA Fragmentation
Detection method	Fluorometric Method
Sample type	Paraffin section, frozen section, cell slide
Assay time	3 hours
Detection instrument	Fluorescence Microscope
Dye Type	Elab Fluor^® 647
Ex/Em (nm)	650/665
Filter set	Cy5
Other reagents required	4% Paraformaldehyde(E-IR-R113),0.2% Triton X-100(E-IR-R122),PBS(E-IR-R187),ddH2O,Mounting solution with anti-fluorescence quencher(E-IR-R119),Xylene,Ethanol
Storage	-20°C, shading light
Shipping	Ice bag
Expiration date	12 months

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Operation Guide

Citations

Nature Communications (2022) IF: 17.694
Long-term monitoring of intravital biological processes using fluorescent protein-assisted NIR-II imaging

DOI: 10.1038/s41467-022-34274-w

PMID: 36333308
DRUG DELIVERY (2022) IF: 6.419
Sevelamer arsenite nanoparticle as a Pi-responsive drug carrier and embolic agent for chemoembolization

DOI: 10.1080/10717544.2022.2072541
Journal of Immunology Research (2022) IF: 4.818
miR-214 Modulates the Growth and Migration of Oral Cancer before and after Chemotherapy through Mediating ULK1

DOI: 10.1155/2022/4589182

PMID: 35692501
RENAL FAILURE (2022) IF: 3.222
Rosiglitazone attenuates hypoxia-induced renal cell apoptosis by inhibiting NF-κB signaling pathway in a PPARγ-dependent manner

DOI: 10.1080/0886022X.2022.2148539
Biopreservation and Biobanking (2021) IF: 2.3
Effects of AavLEA1 Protein on Mouse Ovarian Tissue Cryopreservation by Vitrification

DOI: 10.1089/bio.2021.0048

PMID: 34788107
Journal of Stroke & Cerebrovascular Diseases (2023) IF: 2.677
Inhibition of PI3K/Akt/mTOR signaling by NDRG2 contributes to neuronal apoptosis and autophagy in ischemic stroke

DOI: 10.1016/j.jstrokecerebrovasdis.2023.106984
JOURNAL OF TOXICOLOGICAL SCIENCES (2022) IF: 1.792
Knockdown of UAF1 alleviates sevoflurane-induced cognitive impairment and neurotoxicity in rats by inhibiting pro-inflammatory signaling and oxidative stress

DOI: 10.2131/jts.47.349

PMID: 36047109

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