Biotin Labeling Kit

    • Labeling Kit-Elabscience
    • Labeling Kit-Elabscience
    • Labeling Kit-Elabscience
    <
    >
    • Labeling Kit-Elabscience
    • Labeling Kit-Elabscience
    • Labeling Kit-Elabscience
    • Labeling Kit-Elabscience
    • Labeling Kit-Elabscience
    • Labeling Kit-Elabscience

      Catalog number:E-LK-B002

      Size:
      • 3 reactions
      Qty:
      - +
      Price: $300

      Application: Labeling

      Reactive group: NH2_Reactive Biotin

      Usage: 3 reactions

      Storage: 2-8°C, 12 months

      Lead Time: 7~10 daysWelcome to order from local distributors.

      Add to cart Compare Bulk request Manual

      Introduction

      The Biotin Labeling Kit of Elabscience offering a collection of reagents required for biotin labeling is designed to label antibody with amidogen (NH2-). The biotin in this kit is sufficient and has been activated for direct use. And the reagents are enough for approximately 3 labeling reactions each containing 0.1-2 mg of antibody or other protein. Each kit includes 6 Filtration tubes for desalination of antibody labeling without the need for dialysis. The whole procedure is simple and can be completed in 90 min with proficient operation.

       

      Product Features

      1.  All-inclusive: This kit provides all the reagents required for biotin labeling.

      2.  Quick: The whole procedure takes only 90 min.

      3.  Convenient: Desalination can be achieved with Filtration tube, dialysis or gel filtration is not necessary.

      4.  Flexible: The procedure can be easily adapted to both micro and large scales, with 0.1-2mg of protein labeled each time.

      5.  Perfect results: This kit has been optimized to determine the optimum labeling ratio of biotin to antibody, lowering the possibility of protein inactivation resulted from insufficient labeling or excess biotin labeling.


      Principle

      The NH2-Reactive Biotin reacts with the primary amine (N-terminal and the side chain of lysine residue) specifically, forming stable amide bond





      Assay protocol



      Citations

      1. Functional Roles Of Cadherin, Aminopeptidase-N And Alkaline Phosphatase From Helicoverpa Armigera (Hübner) In The Action Mechanism Of Bacillus Thuringiensis Cry2Aa.
        IF: 4.259
        Journal: Scientific Reports(2017)
        DOI: 10.1038/srep46555
        PMID: 28488696
        Products: E-LK-B002 
      2. Transgenic Cotton Co-Expressing Chimeric Vip3AcAa And Cry1Ac Confers Effective Protection Against Cry1Ac-Resistant Cotton Bollworm.
        IF: 2.341
        Journal: Transgenic Research
        DOI: 10.1007/s11248-017-0048-8
        PMID: 29143178
        Products: E-LK-B002 
      3. Transgenic Cotton Coexpressing Vip3A And Cry1Ac Has A Broad Insecticidal Spectrum Against Lepidopteran Pests.
        IF: 2.379
        Journal: Journal of Invertebrate Pathology(2017)
        DOI: 10.1016/j.jip.2017.08.001
        PMID: 28782511
        Products: E-LK-B002 
      4. FAM19A3, A Novel Secreted Protein, Modulates The Microglia/Macrophage Polarization Dynamics And Ameliorates Cerebral Ischemia.
        IF: 3.623
        Journal: Febs Letters(2015)
        DOI: 10.1016/j.febslet.2015.01.003
        PMID: 25595455
        Products: E-LK-B002 
        Sample type: Protein
        Reactivity: Mouse
      5. New Insights On The Role Of Alkaline Phosphatase 2 From Spodoptera Exigua, (Hübner) In The Action Mechanism Of Bt Toxin Cry2Aa.
        IF: 2.227
        Journal: Journal of Insect Physiology(2017)
        DOI: 10.1016/j.jinsphys.2016.12.004
        PMID: 28034678
        Products: E-LK-B002 
      6. Preparation Of Human Tau Exon-2- And -10-Specific Monoclonal Antibodies For The Recognition Of Brain Tau Proteins In Various Mammals.
        IF: 2.341
        Journal: International Journal of Molecular Medicine(2015)
        DOI: 10.3892/ijmm.2015.2235
        PMID: 26046129
        Products: E-LK-B002 
      7. Ajuba receptor mediates the internalization of tumor-secreted GRP78 into macrophages through different endocytosis pathways.
        IF: 5.168
        Journal: Oncotarget(2018)
        DOI: 10.18632/oncotarget.24090
        PMID: 29643986
        Products: E-LK-F003 E-LK-B002 
      Show AllShorten
      submit review
      • Show all (0)
      • Reviews (0)
      • Q&A (0)
      ... Show All Show Less
      MSDS for ELISA ISO 9001

      Browsing History


        People Also Bought

        Apply for 24T ELISA Kit
        *Product Name:
        *Catalog Number:
        Size:
        *Name:
        *Email:
        *Phone:
        *Message:
        *Country:
        How do you know
        Elabscience:

        *Verification code: