Creatinine (Cr) Colorimetric Assay Kit (Sarcosine Oxidase Method)

    • Biochemical Assay Kit-Elabscience
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    • Biochemical Assay Kit-Elabscience
    • Biochemical Assay Kit-Elabscience

      Catalog number:E-BC-K186-M

      Size:
      • 96T
      • 48T
      Qty:
      - +
      Price: $260
      Can detect 80 samples if without duplication

      Detection method: Colorimetric method

      Lead Time: 7~10 daysWelcome to order from local distributors.

      Add to cart Compare Bulk request Manual

      Detection principle

      Creatinine (Cr) can be catalyzed by sarcosamine hydrolases and generates creatine. Creatine can be hydrolyzed into sarcosine and urea by creatine amine hydrolase. The sarcosine can be catalyzed by sarcosine oxidase and form glycine, formaldehyde and hydrogen peroxide. The reaction between hydrogen peroxide, 2,4-(6-Tri-iodine-3- hydroxybenzoic acid) and 4-ampyrone can be catalyzed by peroxidase and form pink compound. Creatinine content can be calculated indirectly by measuring the OD value at 515 nm.

      Performance characteristics

      Synonyms Cr
      Sensitivity 3.8 μmol/L
      Detection range 27.3-800 μmol/L
      Precision Average inter-assay CV: 3.7%Average intra-assay CV: 1.4%
      Other instruments required Micropipettor, Centrifuge, Incubator, Vortex mixer
      Other reagents required Normal saline (0.9% NaCl)
      Storage 2-8℃
      Valid period 3 months

      Dilution of sample

      It is recommended to take 2~3 samples with expected large difference to do pre-experiment before formal experiment and dilute the sample according to the result of the pre-experiment and the detection range (27.3-800 μmol/L).

      The recommended dilution factor for different samples is as follows (for reference only):

      Sample type

      Dilution factor

      Human urine

      20-30

      Human serum

      1

      Mouse serum

      1

      Rat serum

      1

      Porcine serum

      1

      Note: The diluent is normal saline (0.9% NaCl).

      Citations

      1. Renoprotective effect of irbesartan in a rat model of gentamicin-induced nephrotoxicity: Role of oxidative stress
        Journal: Journal of laboratory physicians(2019)
        DOI: 10.4103/JLP.JLP_136_18
        Products: E-BC-K183-M E-BC-K186-M 
        Sample type: Serum
        Reactivity: Rat
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      MSDS for ELISA ISO 9001

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