Current Position:Home>>Kits>>ELISA Kits>> Human N-MID-OT(N-MID Osteocalcin) ELISA Kit
Cat.No.:E-EL-H0904
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Add to cart Bulk requestAssay type | Sandwich-ELISA |
Format | 96T/48T |
Assay time | 3.5h |
Detection range | 0.63-40 ng/mL |
Sensitivity | 0.38 ng/mL |
Sample type &Sample volume | serum, plasma and other biological fluids; 100μL |
Specificity | This kit recognizes Human N-MID-OT in samples. No significant cross-reactivity or interference between Human N-MID-OT and analogues was observed. |
Reproducibility | Both intra-CV and inter-CV are < 10%. |
Application | This ELISA kit applies to the in vitro quantitative determination of Human N-MID-OT concentrations in serum, plasma and other biological fluids. |
Item | Specifications | Storage |
---|---|---|
Micro ELISA Plate(Dismountable) | 96T: 8 wells ×12 strips
48T: 8 wells ×6 strips |
-20℃, 6 months |
Reference Standard | 96T: 2 vials
48T: 1 vial |
|
Concentrated Biotinylated Detection Ab (100×) | 96T: 1 vial, 120 μL
48T: 1 vial, 60 μL |
|
Concentrated HRP Conjugate (100×) | 96T: 1 vial, 120 μL
48T: 1 vial, 60 μL |
-20℃(Protect from light), 6 months |
Reference Standard & Sample Diluent | 1 vial, 20 mL | 2-8°C, 6 months |
Biotinylated Detection Ab Diluent | 1 vial, 14 mL | |
HRP Conjugate Diluent | 1 vial, 14 mL | |
Concentrated Wash Buffer (25×) | 1 vial, 30 mL | |
Substrate Reagent | 1 vial, 10 mL | 2-8℃(Protect from light) |
Stop Solution | 1 vial, 10 mL | 2-8°C |
Plate Sealer | 5 pieces | |
Manual | 1 copy | |
Certificate of Analysis | 1 copy |
Intra-assay Precision (Precision within an assay): 3 samples with low, mid range and high level Human N-MID-OT were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, mid range and high level Human N-MID-OT were tested on 3 different plates, 20 replicates in each plate.
Intra-assay Precision | Inter-assay Precision | |||||
---|---|---|---|---|---|---|
Sample | 1 | 2 | 3 | 1 | 2 | 3 |
n | 20 | 20 | 20 | 20 | 20 | 20 |
Mean (ng/mL) | 2.16 | 5.18 | 16.62 | 2.37 | 4.68 | 17.80 |
Standard deviation | 0.12 | 0.27 | 0.82 | 0.14 | 0.24 | 0.87 |
CV (%) | 5.56 | 5.21 | 4.93 | 5.91 | 5.13 | 4.89 |
The recovery of Human N-MID-OT spiked at three different levels in samples throughout the range of the assay was evaluated in various matrices.
Sample Type | Range (%) | Average Recovery (%) |
---|---|---|
Serum(n=8) | 94-110 | 101 |
EDTA plasma (n=8) | 93-109 | 100 |
Cell culture media (n=8) | 89-101 | 95 |
Samples were spiked with high concentrations of Human N-MID-OT and diluted with Reference Standard & Sample Diluent to produce samples with values within the range of the assay.
Synonyms | N-MID-OT |
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1. Add 100μL standard or sample to the wells. Incubate for 90 min at 37°C |
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2. Discard the liquid, immediately add 100μL Biotinylated Detection Ab working solution to each well. Incubate for 60 min at 37°C |
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3. Aspirate and wash the plate for 3 times |
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4. Add 100μL HRP conjugate working solution. Incubate for 30 min at 37°C. Aspirate and wash the plate for 5 times |
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5. Add 90μL Substrate Reagent. Incubate for 15 min at 37°C |
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6. Add 50μL Stop Solution |
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7. Read the plate at 450nm immediately. Calculation of the results |
Cat.No. | Product Name | Specifications |
E-ELIR-001 | ELISA Plate Coating Buffer(1×) | 100mL, 1000mL |
E-ELIR-002 | ELISA Plate Coating Buffer(5×) | 100mL, 1000mL |
E-ELIR-003 | ELISA Plate Blocking Buffer | 100mL, 1000mL |
E-ELIR-004 | Wash Buffer for Sandwich-ELISA(25×) | 100mL, 1000mL |
E-ELIR-005 | Wash Buffer for Competitive-ELISA(25×) | 100mL, 1000mL |
E-ELIR-006 | Stop Solution | 100mL, 1000mL |
E-ELIR-007 | HRP-conjugate Stabilizer | 10mL, 100mL, 500mL |
E-ELIR-008 | HRP-conjugate Diluent | 100mL, 1000mL |
E-ELIR-009 | Biotinylated Antibody Stabilizer | 10mL, 100mL, 500mL |
E-ELIR-0010 | Biotinylated Antibody Diluent | 10mL, 100mL, 500mL |
E-ELIR-0011 | Sample Diluent | 10mL, 100mL, 500mL |
From now on, if you have published a paper by using any of our products since 1/1/2019, fill out the “Elabscience Publication Reward Application Form”carefully and send it to orders@elabscience.com, we will get back to you with the reward after we confirm it ASAP!
H***********pSubmitted [ Mar 06 2019 ]
Asked: Hello, are there any experiences in measuring osteocalcin in pig Serum samples. We are searching for an ELISA Kit to measure this. Thanks in Advance for some advice. Heike Bontrup
Reply
adminSubmitted [ Mar 06 2019 ]
Answered: Dear Heike Thanks for your mail. Here we recommend another kit E-EL-P2178 which can measure osteocalcin in pig serum samples. Best regards