Human N-MID-OT(N-MID Osteocalcin) ELISA Kit

This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Human N-MID-OT. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human N-MID-OT and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human N-MID-OT, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Human N-MID-OT. You can calculate the concentration of Human N-MID-OT in the samples by comparing the OD of the samples to the standard curve.

An unopened kit can be stored at 2-8℃ for 1 month. If the kit is not supposed to be used within 1 month, store the components separately according to the following conditions once the kit is received.

Intra-assay Precision (Precision within an assay): 3 samples with low, mid range and high level Human N-MID-OT were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, mid range and high level Human N-MID-OT were tested on 3 different plates, 20 replicates in each plate.

The recovery of Human N-MID-OT spiked at three different levels in samples throughout the range of the assay was evaluated in various matrices.

Elabscience^® provides ancillary reagents required for ELISA kit developments.11 reagents in total, including reagents for coating, blocking, washing, improving the stability and reducing matrix interference etc.