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Mouse IL-12 p40(Interleukin 12 p40) ELISA Kit

  • Cat.No.:E-EL-M2451

  • Reactivity: Mouse

To Purchase E-EL-M2451

  • 96T
  • 48T
  • 24T
  • 96T*5
  • 96T*10
Price: $609

Product Details


Assay type Sandwich-ELISA
Format 96T/48T
Assay time 4.5h
Detection range 15.63-1000 pg/mL
Sensitivity 9.38 pg/mL
Sample type &Sample volume serum, plasma and other biological fluids; 100μL
Specificity This kit recognizes Mouse IL-12 p40 in samples. No significant cross-reactivity or interference between Mouse IL-12 p40 and analogues was observed.
Reproducibility Both intra-CV and inter-CV are < 10%.
Application This ELISA kit applies to the in vitro quantitative determination of Mouse IL-12 p40 concentrations in serum, plasma and other biological fluids.

Test Principle

This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Mouse IL-12 p40. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Mouse IL-12 p40 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Mouse IL-12 p40, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Mouse IL-12 p40. You can calculate the concentration of Mouse IL-12 p40 in the samples by comparing the OD of the samples to the standard curve.

Kit components & Storage

An unopened kit can be stored at 2-8℃ for 1 month. If the kit is not supposed to be used within 1 month, store the components separately according to the following conditions once the kit is received.
Micro ELISA Plate(Dismountable) 96T: 8 wells ×12 strips
48T: 8 wells ×6 strips
-20℃, 6 months
Reference Standard 96T: 2 vials
48T: 1 vial
Concentrated Biotinylated Detection Ab (100×) 96T: 1 vial, 120 μL
48T: 1 vial, 60 μL
Concentrated HRP Conjugate (100×) 96T: 1 vial, 120 μL
48T: 1 vial, 60 μL
-20℃(Protect from light), 6 months
Reference Standard & Sample Diluent 1 vial, 20 mL 2-8°C, 6 months
Biotinylated Detection Ab Diluent 1 vial, 14 mL
HRP Conjugate Diluent 1 vial, 14 mL
Concentrated Wash Buffer (25×) 1 vial, 30 mL
Substrate Reagent 1 vial, 10 mL 2-8℃(Protect from light)
Stop Solution 1 vial, 10 mL 2-8°C
Plate Sealer 5 pieces
Manual 1 copy
Certificate of Analysis 1 copy

Technical Data


Intra-assay Precision (Precision within an assay): 3 samples with low, mid range and high level Mouse IL-12 p40 were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, mid range and high level Mouse IL-12 p40 were tested on 3 different plates, 20 replicates in each plate.

  Intra-assay Precision Inter-assay Precision
Sample 1 2 3 1 2 3
n 20 20 20 20 20 20
Mean (pg/mL) 50.68 116.70 379.42 52.44 116.80 384.10
Standard deviation 2.83 6.02 19.69 2.93 6.03 14.10
CV (%) 5.58 5.16 5.19 5.59 5.16 3.67


The recovery of Mouse IL-12 p40 spiked at three different levels in samples throughout the range of the assay was evaluated in various matrices.

Sample Type Range (%) Average Recovery (%)
Serum(n=8) 89-102 94
EDTA plasma (n=8) 84-96 90
Cell culture media (n=8) 92-107 99

Target Information

Database LinksSwissProt:   P43432
SynonymsIL12B, IL-12B, CLMF2, NKSF2, p40, IL-12/IL-23 p40
Research AreaCancer, Immunology

Assay Procedures

elisa assay procedure 1

1. Add 100μL standard or sample to the wells. Incubate for 90 min at 37°C

elisa assay procedure 2

2. Discard the liquid, immediately add 100μL Biotinylated Detection Ab working solution to each well. Incubate for 60 min at 37°C

elisa assay procedure 3

3. Aspirate and wash the plate for 3 times

elisa assay procedure 4

4. Add 100μL HRP conjugate working solution. Incubate for 30 min at 37°C. Aspirate and wash the plate for 5 times

elisa assay procedure 5

5. Add 90μL Substrate Reagent. Incubate for 15 min at 37°C

elisa assay procedure 6

6. Add 50μL Stop Solution

elisa assay procedure 7

7. Read the plate at 450nm immediately. Calculation of the results


From now on, if you have published a paper by using any of our products since 1/1/2019, fill out the “Elabscience Publication Reward Application Form”carefully and send it to, we will get back to you with the reward after we confirm it ASAP!

View more details about our Publication Reward >>

  1. Cell Death & Disease (2022) IF: 8.4689
    Neurotropic EV71 causes encephalitis by engaging intracellular TLR9 to elicit neurotoxic IL12-p40-iNOS signaling

    DOI: 10.1038/s41419-022-04771-3

    PMID: 35399111

    Sample: serum
  2. Marine Drugs (2022) IF: 5.118
    In Vitro and In Vivo Dendritic Cell Immune Stimulation Effect of Low Molecular Weight Fucoidan from New Zealand Undaria pinnatifida

    DOI: 10.3390/md20030197

    PMID: 35323496

    Sample: Dendritic cells (DCs)
  3. Biomolecules (2020) IF: 4.082
    Preparation, Characterization, and Immuno-Enhancing Activity of Polysaccharides from Glycyrrhiza uralensis

    DOI: 10.3390/biom10010159

    PMID: 31963790

    Sample: Cell culture supernatant,Cell sample
    Comparison of structural characteristics and immunoregulatory activities of polysaccharides from four natural plants

    DOI: 10.1080/09540105.2020.1743647

    Sample: Cell sample,Serum
  5. Journal of Functional Foods (2020) IF: 3.701
    Comparison of the structural characteristics and immunostimulatory activities of polysaccharides from wild and cultivated Pleurotus feruleus

    DOI: 10.1016/j.jff.2020.104050

    Sample: serum
    Exosomes Derived from Human Umbilical Cord Mesenchymal Stem Cells Alleviate Psoriasis-like Skin Inflammation

    DOI: 10.1089/jir.2021.0146

    PMID: 35041513

    Sample: Dendritic cell(DC cell)
  7. Dose-Response (2021) IF: 2.658
    Plasma Proteins As Biodosimetric Markers of Low-Dose Radiation in Mice:

    DOI: 10.1177/15593258211016257

    Sample: Plasma
  8. MOLECULES (2017) IF: 2.861
    Capparis spinosa Fruit Ethanol Extracts Exert Different Effects on the Maturation of Dendritic Cells

    DOI: 10.3390/molecules22010097

    Sample: Cell culture supernatant
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