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Mouse IL-12 p40(Interleukin 12 p40) ELISA Kit

Citations(8)Uniprot : P43432

Cat.No.:E-EL-M2451
Reactivity: Mouse

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Product Details

Properties

Assay type	Sandwich-ELISA
Format	96T/48T
Assay time	4.5h
Detection range	15.63-1000 pg/mL
Sensitivity	9.38 pg/mL
Sample type &Sample volume	serum, plasma and other biological fluids; 100μL
Specificity	This kit recognizes Mouse IL-12 p40 in samples. No significant cross-reactivity or interference between Mouse IL-12 p40 and analogues was observed.
Reproducibility	Both intra-CV and inter-CV are < 10%.
Application	This ELISA kit applies to the in vitro quantitative determination of Mouse IL-12 p40 concentrations in serum, plasma and other biological fluids.

Test Principle

This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Mouse IL-12 p40. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Mouse IL-12 p40 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Mouse IL-12 p40, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Mouse IL-12 p40. You can calculate the concentration of Mouse IL-12 p40 in the samples by comparing the OD of the samples to the standard curve.

Kit components & Storage

An unopened kit can be stored at 2-8℃ for 1 month. If the kit is not supposed to be used within 1 month, store the components separately according to the following conditions once the kit is received.

Item	Specifications	Storage
Micro ELISA Plate(Dismountable)	96T: 8 wells ×12 strips 48T: 8 wells ×6 strips	-20℃, 6 months
Reference Standard	96T: 2 vials 48T: 1 vial
Concentrated Biotinylated Detection Ab (100×)	96T: 1 vial, 120 μL 48T: 1 vial, 60 μL
Concentrated HRP Conjugate (100×)	96T: 1 vial, 120 μL 48T: 1 vial, 60 μL	-20℃(Protect from light), 6 months
Reference Standard & Sample Diluent	1 vial, 20 mL	2-8°C, 6 months
Biotinylated Detection Ab Diluent	1 vial, 14 mL
HRP Conjugate Diluent	1 vial, 14 mL
Concentrated Wash Buffer (25×)	1 vial, 30 mL
Substrate Reagent	1 vial, 10 mL	2-8℃(Protect from light)
Stop Solution	1 vial, 10 mL	2-8°C
Plate Sealer	5 pieces
Manual	1 copy
Certificate of Analysis	1 copy

Technical Data

Precision

Intra-assay Precision (Precision within an assay): 3 samples with low, mid range and high level Mouse IL-12 p40 were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, mid range and high level Mouse IL-12 p40 were tested on 3 different plates, 20 replicates in each plate.

	Intra-assay Precision			Inter-assay Precision
Sample	1	2	3	1	2	3
n	20	20	20	20	20	20
Mean (pg/mL)	50.68	116.70	379.42	52.44	116.80	384.10
Standard deviation	2.83	6.02	19.69	2.93	6.03	14.10
CV (%)	5.58	5.16	5.19	5.59	5.16	3.67

Recovery

The recovery of Mouse IL-12 p40 spiked at three different levels in samples throughout the range of the assay was evaluated in various matrices.

Sample Type	Range (%)	Average Recovery (%)
Serum(n=8)	89-102	94
EDTA plasma (n=8)	84-96	90
Cell culture media (n=8)	92-107	99

Target Information

Database Links	SwissProt: P43432
Synonyms	IL12B, IL-12B, CLMF2, NKSF2, p40, IL-12/IL-23 p40
Research Area	Cancer, Immunology

Assay Procedures

	1. Add 100μL standard or sample to the wells. Incubate for 90 min at 37°C
	2. Discard the liquid, immediately add 100μL Biotinylated Detection Ab working solution to each well. Incubate for 60 min at 37°C
	3. Aspirate and wash the plate for 3 times
	4. Add 100μL HRP conjugate working solution. Incubate for 30 min at 37°C. Aspirate and wash the plate for 5 times
	5. Add 90μL Substrate Reagent. Incubate for 15 min at 37°C
	6. Add 50μL Stop Solution
	7. Read the plate at 450nm immediately. Calculation of the results

Citations

From now on, if you have published a paper by using any of our products since 1/1/2019, fill out the “Elabscience Publication Reward Application Form”carefully and send it to orders@elabscience.com, we will get back to you with the reward after we confirm it ASAP!

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Cell Death & Disease (2022) IF: 8.4689

Neurotropic EV71 causes encephalitis by engaging intracellular TLR9 to elicit neurotoxic IL12-p40-iNOS signaling

DOI: 10.1038/s41419-022-04771-3
PMID: 35399111	Sample: serum

Marine Drugs (2022) IF: 5.118

In Vitro and In Vivo Dendritic Cell Immune Stimulation Effect of Low Molecular Weight Fucoidan from New Zealand Undaria pinnatifida

DOI: 10.3390/md20030197
PMID: 35323496	Sample: Dendritic cells (DCs)

Biomolecules (2020) IF: 4.082

Preparation, Characterization, and Immuno-Enhancing Activity of Polysaccharides from Glycyrrhiza uralensis

DOI: 10.3390/biom10010159
PMID: 31963790	Sample: Cell culture supernatant,Cell sample

FOOD AND AGRICULTURAL IMMUNOLOGY (2020) IF: 3.101

Comparison of structural characteristics and immunoregulatory activities of polysaccharides from four natural plants

DOI: 10.1080/09540105.2020.1743647
	Sample: Cell sample,Serum

Journal of Functional Foods (2020) IF: 3.701

Comparison of the structural characteristics and immunostimulatory activities of polysaccharides from wild and cultivated Pleurotus feruleus

DOI: 10.1016/j.jff.2020.104050
	Sample: serum

JOURNAL OF INTERFERON AND CYTOKINE RESEARCH (2022) IF: 2.607

Exosomes Derived from Human Umbilical Cord Mesenchymal Stem Cells Alleviate Psoriasis-like Skin Inflammation

DOI: 10.1089/jir.2021.0146
PMID: 35041513	Sample: Dendritic cell(DC cell)

Dose-Response (2021) IF: 2.658

Plasma Proteins As Biodosimetric Markers of Low-Dose Radiation in Mice:

DOI: 10.1177/15593258211016257
	Sample: Plasma

MOLECULES (2017) IF: 2.861

Capparis spinosa Fruit Ethanol Extracts Exert Different Effects on the Maturation of Dendritic Cells

DOI: 10.3390/molecules22010097
	Sample: Cell culture supernatant

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