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Proline (Pro) Colorimetric Assay Kit

  • Cat.No.:E-BC-K177-M

  • Detection instrument: Microplate reader (520 nm)

To Purchase E-BC-K177-M

Size:
  • 96T
  • 48T
Price: $360
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Detection principle

Proline can react with acidic-ninhydrin to form stable red compound. The maximum absorption peak of the compound is at 520 nm. And the concentration of Pro can be calculated by measuring the OD value at 520 nm

Performance characteristics

Synonyms Pro
Sample type plant tissue and honey sample
Sensitivity 0.57 μg/mL
Detection range 0.57-35 μg/mL
Detection method Colorimetric method
Assay type Quantitative
Precision Average inter-assay CV: 4.7-10.0%Average intra-assay CV: 3.7-5.5%
Other instruments required Vortex mixer, Water bath, Centrifuge
Other reagents required Acetic acid
Storage 2-8℃
Valid period 12 months

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