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Rat ANP(Atrial Natriuretic Peptide) ELISA Kit

Cat.No.:E-EL-R0017
Reactivity: Rat

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Product Details

Properties

Assay type	Competitive-ELISA
Format	96T/48T
Assay time	2.0h
Detection range	15.63-1000 pg/mL
Sensitivity	9.38 pg/mL
Sample type &Sample volume	serum, plasma and other biological fluids; 50μL
Specificity	This kit recognizes ANP in samples. No significant cross-reactivity or interference between ANP and analogues was observed.
Reproducibility	Both intra-CV and inter-CV are < 10%.
Application	This ELISA kit applies to the in vitro quantitative determination of ANP concentrations in serum, plasma and other biological fluids.

Test Principle

This ELISA kit uses the Competitive-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with ANP. During the reaction, ANP in the sample or standard competes with a fixed amount of ANP on the solid phase supporter for sites on the Biotinylated Detection Ab specific to ANP. Excess conjugate and unbound sample or standard are washed away, and Avidin-Horseradish Peroxidase (HRP) conjugate are added to each micro plate well and incubated. Then a TMB substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns from blue to yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of ANP in tested samples can be calculated by comparing the OD of the samples to the standard curve.

Kit components & Storage

An unopened kit can be stored at 2-8℃ for 1 month. If the kit is not supposed to be used within 1 month, store the components separately according to the following conditions once the kit is received.

Item	Specifications	Storage
Micro ELISA Plate(Dismountable)	96T: 8 wells ×12 strips 48T: 8 wells ×6 strips	-20℃, 6 months
Reference Standard	96T: 2 vials 48T: 1 vial
Concentrated Biotinylated Detection Ab (100×)	96T: 1 vial, 120 μL 48T: 1 vial, 60 μL
Concentrated HRP Conjugate (100×)	96T: 1 vial, 120 μL 48T: 1 vial, 60 μL	-20℃(Protect from light), 6 months
Reference Standard & Sample Diluent	1 vial, 20 mL	2-8°C, 6 months
Biotinylated Detection Ab Diluent	1 vial, 14 mL
HRP Conjugate Diluent	1 vial, 14 mL
Concentrated Wash Buffer (25×)	1 vial, 30 mL
Substrate Reagent	1 vial, 10 mL	2-8℃(Protect from light)
Stop Solution	1 vial, 10 mL	2-8℃
Plate Sealer	5 pieces
Manual	1 copy
Certificate of Analysis	1 copy

Technical Data

Precision

Intra-assay Precision (Precision within an assay): 3 samples with low, mid range and high level ANP were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, mid range and high level ANP were tested on 3 different plates, 20 replicates in each plate.

	Intra-assay Precision			Inter-assay Precision
Sample	1	2	3	1	2	3
n	20	20	20	20	20	20
Mean (pg/mL)	55.20	123.50	465.90	58.30	133.70	440.90
Standard deviation	3.90	5.40	16.30	3.40	6.30	23.80
CV (%)	7.07	4.37	3.50	5.83	4.71	5.40

Recovery

The recovery of ANP spiked at three different levels in samples throughout the range of the assay was evaluated in various matrices.

Sample Type	Range (%)	Average Recovery (%)
Serum(n=8)	89-104	95
EDTA plasma(n=8)	94-107	99
Cell culture media(n=8)	87-102	93

Linearity

Samples were spiked with high concentrations of Rat ANP and diluted with Reference Standard & Sample Diluent to produce samples with values within the range of the assay.

Target Information

Synonyms	NPPA, ANF, ANH, ATFB6, CDD-ANF, PND, Atriopeptin
Research Area	Cancer, Cardiovascular, Signal transduction

Assay Procedures

	1. Add 50μL standard or sample to the wells, immediately add 50μL Biotinylated Detection Ab working solution to each well. Incubate for 45 min at 37°C
	2. Aspirate and wash the plate for 3 times
	3. Add 100μL HRP conjugate working solution. Incubate for 30 min at 37°C. Aspirate and wash the plate for 5 times
	4. Add 90μL Substrate Reagent. Incubate for 15 min at 37°C
	5. Add 50μL Stop Solution
	6. Read the plate at 450nm immediately. Calculation of the results

Citations

From now on, if you have published a paper by using any of our products since 1/1/2019, fill out the “Elabscience Publication Reward Application Form”carefully and send it to orders@elabscience.com, we will get back to you with the reward after we confirm it ASAP!

View more details about our Publication Reward >>

INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES (2022) IF: 6.208

Natriuretic Peptides—New Targets for Neurocontrol of Blood Pressure via Baroreflex Afferent Pathway

DOI: 10.3390/ijms232113619
	Sample: nucleus tractus solitarius (NTS) tissue

BIOMEDICINE & PHARMACOTHERAPY (2021) IF: 6.53

Water-soluble alkaloids extracted from Aconiti Radix lateralis praeparata protect against chronic heart failure in rats via a calcium signaling pathway

DOI: 10.1016/j.biopha.2020.111184
PMID: 33418305	Sample: Plasma

JOURNAL OF CELLULAR PHYSIOLOGY (2020) IF: 5.546

Autophagy decreases alveolar epithelial cell injury by regulating the release of inflammatory mediators

DOI: 10.1002/jcp.29453
PMID: 31960959	Sample: Cell culture supernatant,Tissue homogenate

Cell Death & Disease (2017) IF: 5.965

ANP promotes proliferation and inhibits apoptosis of ovarian granulosa cells by NPRA/PGRMC1/EGFR complex and improves ovary functions of PCOS rats

DOI: 10.1038/cddis.2017.494
	Sample: Serum

TOXICOLOGY IN VITRO (2018) IF: 3.105

Glucotoxicity results in apoptosis in H9c2 cells via alteration in redox homeostasis linked mitochondrial dynamics and polyol pathway and possible reversal with cinnamic acid

DOI: 10.1016/j.tiv.2018.08.010
PMID: 30144576	Sample: Cell culture supernatant

BIOMEDICINE & PHARMACOTHERAPY (2018) IF: 3.457

Chlorogenic acid attenuates glucotoxicity in H9c2 cells via inhibition of glycation and PKC α upregulation and safeguarding innate antioxidant status

DOI: 10.1016/j.biopha.2018.02.027
	Sample: Cell culture supernatant

VOX SANGUINIS (2021) IF: 2.144

Differential effects of speed and volume on transfusion-associated circulatory overload: A randomized study in rats

DOI: 10.1111/vox.13191
PMID: 34396543	Sample: serum

FUNDAMENTAL & CLINICAL PHARMACOLOGY (2019) IF: 2.229

High glucose induced calcium overload via impairment of SERCA/PLN pathway and mitochondrial dysfunction leads to oxidative stress in H9c2 cells and amelioration with ferulic acid

DOI: 10.1111/fcp.12452
PMID: 30739350	Sample: Cell culture medium

JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES (2019) IF: 2.813

Synergistic effect of Aconiti Lateralis Radix Praeparata water-soluble alkaloids and Ginseng Radix et Rhizoma total ginsenosides compatibility on acute heart failure rats

DOI: 10.1016/j.jchromb.2019.121935
PMID: 31877430	Sample: Serum

Natural Product Communications (2023) IF: 1.496

Vanillic Acid Alleviates Right Ventricular Function in Rats With MCT-Induced Pulmonary Arterial Hypertension

DOI: 10.1177/1934578X221148896
	Sample: serum

Experimental and Therapeutic Medicine (2020) IF: 1.785

Exendin‑4 inhibits atrial arrhythmogenesis in a model of myocardial infarction‑induced heart failure via the GLP‑1 receptor signaling pathway

DOI: 10.3892/etm.2020.9089
	Sample: Plasma

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