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Rat FETUA(Fetuin A) ELISA Kit

Cat.No.:E-EL-R2451
Reactivity: Rat

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Product Details

Properties

Assay type	Sandwich-ELISA
Format	96T/48T
Assay time	3.5h
Detection range	6.25-400 ng/mL
Sensitivity	3.75 ng/mL
Sample type &Sample volume	serum, plasma and other biological fluids; 100μL
Specificity	This kit recognizes Rat FETUA in samples. No significant cross-reactivity or interference between Rat FETUA and analogues was observed.
Reproducibility	Both intra-CV and inter-CV are < 10%.
Application	This ELISA kit applies to the in vitro quantitative determination of Rat FETUA concentrations in serum, plasma and other biological fluids.

Test Principle

This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Rat FETUA. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Rat FETUA and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Rat FETUA, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Rat FETUA. You can calculate the concentration of Rat FETUA in the samples by comparing the OD of the samples to the standard curve.

Kit components & Storage

An unopened kit can be stored at 2-8℃ for 1 month. If the kit is not supposed to be used within 1 month, store the components separately according to the following conditions once the kit is received.

Item	Specifications	Storage
Micro ELISA Plate(Dismountable)	96T: 8 wells ×12 strips 48T: 8 wells ×6 strips	-20℃, 6 months
Reference Standard	96T: 2 vials 48T: 1 vial
Concentrated Biotinylated Detection Ab (100×)	96T: 1 vial, 120 μL 48T: 1 vial, 60 μL
Concentrated HRP Conjugate (100×)	96T: 1 vial, 120 μL 48T: 1 vial, 60 μL	-20℃(Protect from light), 6 months
Reference Standard & Sample Diluent	1 vial, 20 mL	2-8°C, 6 months
Biotinylated Detection Ab Diluent	1 vial, 14 mL
HRP Conjugate Diluent	1 vial, 14 mL
Concentrated Wash Buffer (25×)	1 vial, 30 mL
Substrate Reagent	1 vial, 10 mL	2-8℃(Protect from light)
Stop Solution	1 vial, 10 mL	2-8°C
Plate Sealer	5 pieces
Manual	1 copy
Certificate of Analysis	1 copy

Technical Data

Precision

Intra-assay Precision (Precision within an assay): 3 samples with low, mid range and high level Rat FETUA were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, mid range and high level Rat FETUA were tested on 3 different plates, 20 replicates in each plate.

	Intra-assay Precision			Inter-assay Precision
Sample	1	2	3	1	2	3
n	20	20	20	20	20	20
Mean (ng/mL)	21.49	52.33	177.04	22.43	49.95	190.26
Standard deviation	1.21	2.86	6.59	1.43	2.53	9.97
CV (%)	5.63	5.47	3.72	6.38	5.07	5.24

Recovery

The recovery of Rat FETUA spiked at three different levels in samples throughout the range of the assay was evaluated in various matrices.

Sample Type	Range (%)	Average Recovery (%)
Serum(n=8)	93-107	98
EDTA plasma (n=8)	92-105	99
Cell culture media (n=8)	95-110	100

Linearity

Samples were spiked with high concentrations of Rat FETUA and diluted with Reference Standard & Sample Diluent to produce samples with values within the range of the assay.

Target Information

Synonyms	AHSG, A2HS, AHS, HSGA, alpha-2-HS-glycoprotein
Research Area	Cardiovascular, Metabolism, Neuroscience

Assay Procedures

	1. Add 100μL standard or sample to the wells. Incubate for 90 min at 37°C
	2. Discard the liquid, immediately add 100μL Biotinylated Detection Ab working solution to each well. Incubate for 60 min at 37°C
	3. Aspirate and wash the plate for 3 times
	4. Add 100μL HRP conjugate working solution. Incubate for 30 min at 37°C. Aspirate and wash the plate for 5 times
	5. Add 90μL Substrate Reagent. Incubate for 15 min at 37°C
	6. Add 50μL Stop Solution
	7. Read the plate at 450nm immediately. Calculation of the results

Citations

From now on, if you have published a paper by using any of our products since 1/1/2019, fill out the “Elabscience Publication Reward Application Form”carefully and send it to orders@elabscience.com, we will get back to you with the reward after we confirm it ASAP!

View more details about our Publication Reward >>

Aging Cell (2021) IF: 9.304

Spermidine inhibits vascular calcification in chronic kidney disease through modulation of SIRT1 signaling pathway

DOI: 10.1111/acel.13377
PMID: 33969611	Sample: Serum

Reviews/Q&A

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Reviews
Q&A

M*****aSubmitted [ Jun 29 2022 ]

Asked: I received email that says \"your question about the product Rat FETUA(Fetuin A) ELISA Kit has been replied\" but I don\'t know how to check the answer. Please tell me how to do it.

adminSubmitted [ Jun 29 2022 ]

Answered: We have received your feedback about the problem. The problem of no signal in the sample you complained belongs to the after-sales problem. We hope you can fill in the after-sales registration form to help us understand the relevant information. We would be grateful if you could provide additional sample information. 1. Are your rat serum samples diluted for testing? 2. How are your rat serum samples collected and preserved? How long did it last? 3. What is the disease model you are studying? We will send a sheet by e-mail. Pls kindly check it.

K*********iSubmitted [ Jun 29 2022 ]

Asked: I tried to calculate fetuin-a concentration in rat serum by using this kit. Although signals of standard sample was detected signals of rat serum was not detected at all. Could you tell me your thought of the reason why signals of rat serum was not detected?

adminSubmitted [ Jun 29 2022 ]

Answered: Please obtain the after-sales registration form from the dealer who sold the kit to you and fill in the kit number, lot number, relevant experimental operation information and original data of the kit. We will help you solve the corresponding problems later.

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